大鼠初次抗体应答过程中抗原特异性分泌IgE细胞的动力学及分布
Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat.
作者信息
Sedgwick J D, Holt P G
出版信息
J Exp Med. 1983 Jun 1;157(6):2178-83. doi: 10.1084/jem.157.6.2178.
Abstract
A new assay system is described for the enumeration of antigen-specific IgE immunoglobulin-secreting cells (ISC) based on an enzyme-linked immunoabsorbent assay. Using this technique to monitor the organ distribution of OVA-specific ISC after primary immunization of rats, approximately 12,000 specific IgE ISC were detected at the peak of the response in the draining lymph nodes compared with 117,000 IgG ISC; the splenic anti-OVA response was restricted to the IgM class. Using plates precoated with anti-rat IgE instead of antigen, total IgE ISC were enumerated in normal and helminth-parasitized rats. The assay system detected up to 5 X 10(5) IgE ISC in mesenteric lymph nodes from parasitized animals compared with less than 50 in controls.
摘要
描述了一种基于酶联免疫吸附测定法来计数抗原特异性IgE免疫球蛋白分泌细胞（ISC）的新检测系统。使用该技术监测大鼠初次免疫后OVA特异性ISC的器官分布，在引流淋巴结反应高峰期检测到约12,000个特异性IgE ISC，而IgG ISC为117,000个；脾脏抗OVA反应仅限于IgM类。使用预包被抗大鼠IgE而非抗原的平板，对正常和感染蠕虫的大鼠中的总IgE ISC进行计数。该检测系统在感染动物的肠系膜淋巴结中检测到多达5×10⁵个IgE ISC，而对照组中少于50个。
相似文献
1
Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat.
J Exp Med. 1983 Jun 1;157(6):2178-83. doi: 10.1084/jem.157.6.2178.
2
Induction of IgE-secreting cells and IgE isotype-specific suppressor T cells in the respiratory lymph nodes of rats in response to antigen inhalation.
Cell Immunol. 1985 Aug;94(1):182-94. doi: 10.1016/0008-8749(85)90095-4.
3
The enumeration of rat IgE-secreting cells using a reverse plaque-forming cell assay.
Eur J Immunol. 1979 Jun;9(6):471-6. doi: 10.1002/eji.1830090611.
4
Potentiated IgE response in Nippostrongylus brasiliensis infested rats--sites of synthesis and traffic of cells secreting potentiated antibody.
Int Arch Allergy Appl Immunol. 1986;81(2):97-103. doi: 10.1159/000234116.
5
Development of IgE-forming cells in vitro from rat mesenteric lymph node cells.
J Immunol. 1978 Dec;121(6):2413-21.
6
Regulation of antigen-specific versus by-stander IgE production after antigen sensitization.
Cell Immunol. 2004 Jun;229(2):106-16. doi: 10.1016/j.cellimm.2004.06.007.
7
Induction of IgE-secreting cells in the lymphatic drainage of the lungs of rats following passive antigen inhalation.
Int Arch Allergy Appl Immunol. 1986;79(3):329-31. doi: 10.1159/000233995.
8
Nature of cells binding anti-IgE in rats immunized with Nippostrongylus brasiliensis: IgE synthesis in regional nodes and concentration in mucosal mast cells.
Eur J Immunol. 1976 Aug;6(8):537-45. doi: 10.1002/eji.1830060803.
9
The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
Toxicology. 2005 Jan 15;206(2):181-93. doi: 10.1016/j.tox.2004.07.018.
10
IgE antibody-forming cells in rats infected with Nippostrongylus brasiliensis and immunized with antigens.
Cell Immunol. 1988 Sep;115(2):460-70. doi: 10.1016/0008-8749(88)90198-0.
引用本文的文献
1
Combining magnetically isolated CD45 cells with serum maintains intact drug responsiveness for ELISpot analysis in clinical trials.
Immunohorizons. 2025 Jan 27;9(3). doi: 10.1093/immhor/vlae012.
2
Differential immune cell infiltrations between healthy periodontal and chronic periodontitis tissues.
BMC Oral Health. 2020 Oct 27;20(1):293. doi: 10.1186/s12903-020-01287-0.
3
The regulated long-term delivery of therapeutic proteins by using antigen-specific B lymphocytes.
Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16298-303. doi: 10.1073/pnas.0405271101. Epub 2004 Nov 1.
4
Detection and characterization of plasma cells in peripheral blood: correlation of IgE+ plasma cell frequency with IgE serum titre.
Clin Exp Immunol. 2002 Dec;130(3):370-8. doi: 10.1046/j.1365-2249.2002.02025.x.
5
Ricin enhances IgE responses by inhibiting a subpopulation of early-activated IgE regulatory CD8+ T cells.
Immunology. 1993 Feb;78(2):226-36.
6
Role of antibody and T cells in the long-term inhibition of IgE synthesis.
Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):604-8. doi: 10.1073/pnas.91.2.604.
7
Down-regulation of immune responses to inhaled antigen: studies on the mechanism of induced suppression.
Immunology. 1985 Dec;56(4):635-42.
8
Can persistent IgE responses be suppressed?
Clin Exp Immunol. 1990 Dec;82(3):423-6. doi: 10.1111/j.1365-2249.1990.tb05464.x.
9
Inhibition of IgE synthesis by anti-IgE: role in long-term inhibition of IgE synthesis by neonatally administered soluble IgE.
Proc Natl Acad Sci U S A. 1990 May;87(9):3363-7. doi: 10.1073/pnas.87.9.3363.
10
IgE-secreting cells in the thymus: correlation with induction of tolerance to IgE.
Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5185-7. doi: 10.1073/pnas.89.11.5185.
本文引用的文献
1
A clonal analysis of the IgE response and its implications with regard to isotope commitment.
J Exp Med. 1981 Apr 1;153(4):783-92. doi: 10.1084/jem.153.4.783.
2
Recent studies on the regulation of IgE antibody synthesis in experimental animals and man.
Immunology. 1980 Sep;41(1):1-24.
3
A solid-phase immunoenzymatic technique for the enumeration of specific antibody-secreting cells.
J Immunol Methods. 1983 Feb 25;57(1-3):301-9. doi: 10.1016/0022-1759(83)90091-1.
4
Patterns of lymphatic drainage in the adult laboratory rat.
J Anat. 1971 Sep;109(Pt 3):369-83.
5
Communications. Heterologous adoptive cutaneous anaphylaxis: a method for detecting reaginic antibody formation by cells of the mouse.
J Immunol. 1973 Aug;111(2):638-40.
6
Hapten-specific IgE antibody responses in mice. I. Secondary IgE responses in irradiated recipients of syngeneic primed spleen cells.
J Exp Med. 1973 Jul 1;138(1):306-11. doi: 10.1084/jem.138.1.306.
7
Regulation of homocytotropic antibody formation in the rat. 3. Effect of thymectomy and splenectomy.
J Immunol. 1971 Apr;106(4):1019-25.
8
Distribution of gamma E-forming cells in lymphoid tissues of the human and monkey.
J Immunol. 1970 Feb;104(2):377-87.
9
10
Immunoglobulin E (IgE) and IgE-containing cells in human gastrointestinal fluids and tissues.
Clin Exp Immunol. 1975 May;20(2):227-37.
Zotero 插件