大鼠初次抗体应答过程中抗原特异性分泌IgE细胞的动力学及分布
Kinetics and distribution of antigen-specific IgE-secreting cells during the primary antibody response in the rat.
作者信息
Sedgwick J D, Holt P G
出版信息
J Exp Med. 1983 Jun 1;157(6):2178-83. doi: 10.1084/jem.157.6.2178.
Abstract
A new assay system is described for the enumeration of antigen-specific IgE immunoglobulin-secreting cells (ISC) based on an enzyme-linked immunoabsorbent assay. Using this technique to monitor the organ distribution of OVA-specific ISC after primary immunization of rats, approximately 12,000 specific IgE ISC were detected at the peak of the response in the draining lymph nodes compared with 117,000 IgG ISC; the splenic anti-OVA response was restricted to the IgM class. Using plates precoated with anti-rat IgE instead of antigen, total IgE ISC were enumerated in normal and helminth-parasitized rats. The assay system detected up to 5 X 10(5) IgE ISC in mesenteric lymph nodes from parasitized animals compared with less than 50 in controls.
摘要
描述了一种基于酶联免疫吸附测定法来计数抗原特异性IgE免疫球蛋白分泌细胞（ISC）的新检测系统。使用该技术监测大鼠初次免疫后OVA特异性ISC的器官分布，在引流淋巴结反应高峰期检测到约12,000个特异性IgE ISC，而IgG ISC为117,000个；脾脏抗OVA反应仅限于IgM类。使用预包被抗大鼠IgE而非抗原的平板，对正常和感染蠕虫的大鼠中的总IgE ISC进行计数。该检测系统在感染动物的肠系膜淋巴结中检测到多达5×10⁵个IgE ISC，而对照组中少于50个。
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