Department of Stomatology, Beijing Chao-Yang Hospital, Capital Medical University, 8th Gongti South Road, Beijing, 100020, China.
Department of Periodontology, Tianjin Stomatological Hospital, Tianjin Key Laboratory of Oral Function Reconstruction, Hospital of Stomatology, Nankai University, 75th Dagu North Road, Tianjin, 300000, China.
BMC Oral Health. 2020 Oct 27;20(1):293. doi: 10.1186/s12903-020-01287-0.
Host immunity plays an important role against oral microorganisms in periodontitis.
This study assessed the infiltrating immune cell subtypes in 133 healthy periodontal and 210 chronic periodontitis tissues from Gene Expression Omnibus (GEO) datasets using the CIBERSORT gene signature files.
Plasma cells, naive B cells and neutrophils were all elevated in periodontitis tissues, when compared to those in healthy controls. In contrast, memory B cells, resting dendritic, mast cells and CD4 memory cells, as well as activated mast cells, M1 and M2 macrophages, and follicular helper T cells, were mainly present in healthy periodontal tissues. Furthermore, these periodontitis tissues generally contained a higher proportion of activated CD4 memory T cells, while the other subtypes of T cells, including resting CD4 memory T cells, CD8 T cells, follicular helper T cells (T) and regulatory T cells (Tregs), were relatively lower in periodontitis tissues, when compared to healthy tissues. The ratio of dendritic and mast cells and macrophages was lower in periodontitis tissues, when compared to healthy tissues. In addition, there was a significant negative association of plasma cells with most of the other immune cells, such as plasma cells vs. memory B cells (γ = - 0.84), plasma cells vs. resting dendritic cells (γ = - 0.64), plasma cells vs. resting CD4 memory T cells (γ = 0.50), plasma cells versus activated dendritic cells (γ = - 0.46), plasma cells versus T (γ = - 0.46), plasma cells versus macrophage M2 cells (γ = - 0.43), or plasma cells versus macrophage M1 cells (γ = - 0.40), between healthy control and periodontitis tissues.
Plasma cells, naive B cells and neutrophils were all elevated in periodontitis tissues. The infiltration of different immune cell subtypes in the periodontitis site could lead the host immunity against periodontitis.
宿主免疫在牙周炎中对口腔微生物发挥着重要作用。
本研究使用 CIBERSORT 基因特征文件，从基因表达综合数据库（GEO）数据集评估了 133 例健康牙周组织和 210 例慢性牙周炎组织中的浸润免疫细胞亚群。
与健康对照组相比，浆细胞、幼稚 B 细胞和中性粒细胞在牙周炎组织中均升高。相比之下，记忆 B 细胞、静息树突状细胞、肥大细胞和 CD4 记忆细胞以及激活的肥大细胞、M1 和 M2 巨噬细胞以及滤泡辅助 T 细胞主要存在于健康牙周组织中。此外，这些牙周炎组织通常含有更高比例的活化 CD4 记忆 T 细胞，而其他类型的 T 细胞，包括静息 CD4 记忆 T 细胞、CD8 T 细胞、滤泡辅助 T 细胞（T）和调节性 T 细胞（Treg），在牙周炎组织中相对较低，与健康组织相比。与健康组织相比，树突状细胞和肥大细胞以及巨噬细胞的比例在牙周炎组织中较低。此外，浆细胞与大多数其他免疫细胞呈显著负相关，如浆细胞与记忆 B 细胞（γ=-0.84）、浆细胞与静息树突状细胞（γ=-0.64）、浆细胞与静息 CD4 记忆 T 细胞（γ=0.50）、浆细胞与活化树突状细胞（γ=-0.46）、浆细胞与 T（γ=-0.46）、浆细胞与 M2 巨噬细胞（γ=-0.43）或浆细胞与 M1 巨噬细胞（γ=-0.40）之间存在显著负相关，无论是在健康对照组还是牙周炎组织中。
浆细胞、幼稚 B 细胞和中性粒细胞在牙周炎组织中均升高。牙周炎部位不同免疫细胞亚群的浸润可能导致宿主对牙周炎的免疫反应。
