Husby G, Blomhoff J P, Elgjo K, Williams R C
Scand J Gastroenterol. 1982 Oct;17(7):855-60. doi: 10.3109/00365528209181105.
Liver biopsies from 27 patients were studied by the indirect immunofluorescence and immunoperoxidase technique for relative distribution of B cells and T-cell subpopulations. T-cell subsets were defined for OKT4(+) (helper/inducer) and OKT8(+) (suppressor/cytotoxic) cells by using mouse hybridoma monoclonal antibodies. Patients with chronic active hepatitis and those with primary biliary cirrhosis showed marked relative increments in suppressor/cytotoxic OKT8(+) lymphocytes within hepatic lymphocytic infiltrates. Other patients with acute hepatitis or those with only reactive changes also showed a relative increment in OKT8(+) T lymphocytes within hepatic biopsy material. These findings suggest that tissue lymphocytic subpopulation increments in suppressor/cytotoxic T-cell subpopulations are commonly associated with hepatic lymphocytic infiltrates and may be related to disorders of immune regulation or expression in some of these disorders.
采用间接免疫荧光和免疫过氧化物酶技术，对27例患者的肝活检组织进行研究，以观察B细胞和T细胞亚群的相对分布情况。通过使用小鼠杂交瘤单克隆抗体，将T细胞亚群定义为OKT4(+)（辅助/诱导）细胞和OKT8(+)（抑制/细胞毒性）细胞。慢性活动性肝炎患者和原发性胆汁性肝硬化患者的肝淋巴细胞浸润区域内，抑制/细胞毒性OKT8(+)淋巴细胞显著相对增多。急性肝炎患者或仅有反应性改变的患者，其肝活检组织内的OKT8(+) T淋巴细胞也相对增多。这些发现表明，抑制/细胞毒性T细胞亚群的组织淋巴细胞亚群增多通常与肝淋巴细胞浸润有关，并且可能与这些疾病中的免疫调节紊乱或表达有关。
