Koropatnick J, Andrews G, Duerksen J D, Varshney U, Gedamu L
Nucleic Acids Res. 1983 May 25;11(10):3255-67. doi: 10.1093/nar/11.10.3255.
Micrococcal nuclease has been shown to preferentially cleave chromatin in the region of genes actively engaged in transcription. We have used this preferential cleavage to show that the metallothionein (MT) gene in adult mouse liver, when induced to produce mRNA by injection of cadmium, becomes more susceptible to nuclease cleavage. However, the MT gene in uninduced liver, and the alphafoetal protein (AFP) gene in both induced and uninduced liver, remain relatively resistant to nuclease cleavage. The AFP gene is not normally expressed in cadmium induced or uninduced liver. Thus, susceptibility of genes to nuclease cleavage appears to rise with increasing transcription of the gene.
已证明微球菌核酸酶可优先切割活跃参与转录的基因区域中的染色质。我们利用这种优先切割来表明，成年小鼠肝脏中的金属硫蛋白（MT）基因在通过注射镉诱导产生mRNA时，变得更容易被核酸酶切割。然而，未诱导肝脏中的MT基因以及诱导和未诱导肝脏中的甲胎蛋白（AFP）基因对核酸酶切割仍具有相对抗性。AFP基因在镉诱导或未诱导的肝脏中通常不表达。因此，基因对核酸酶切割的敏感性似乎随着基因转录的增加而升高。
