Chrzanowski Grzegorz, Pasternak Grzegorz, Aebisher David, Dynarowicz Klaudia, Myśliwiec Angelika, Bartusik-Aebisher Dorota, Sosna Barbara, Cieślar Grzegorz, Kawczyk-Krupka Aleksandra, Filip Rafał
Department of Biology, College of Natural Sciences, University of Rzeszów, 35-310 Rzeszów, Poland.
Department of General Surgery, Provincial Clinical Hospital No. 2 in Rzeszów, 35-301 Rzeszów, Poland.
Life (Basel). 2023 Oct 5;13(10):2013. doi: 10.3390/life13102013.
One of the inflammatory bowel diseases is Crohn's disease. Although this term has been used in the medical community since 1932, a significant increase in the number of publications occurs at the end of the 20th century and the beginning of the 21st century. Crohn's disease is a disease that cannot be fully cured. In many cases, it is chronic, i.e., recurrent. All preventive and therapeutic measures taken by doctors are aimed at inhibiting the development of the disease and minimizing the occurrence of any potential "side effects" resulting from the developing disease. One of the diagnostic methods is the qualitative and quantitative determination of metalloproteinases in inflammatory tissues and in the blood. The aim of the study was the quantitative and qualitative determination of metalloproteinases in inflammatory bowel tissues in patients diagnosed with Crohn's disease. The in vitro study was performed on surgical tissues from patients diagnosed with Crohn's disease. The results show that in inflammatory tissues the concentration of metalloproteinases -3, -7, -8, -9 was higher compared to tissues taken from the resection margin without signs of inflammation, defined as healthy. The experiment confirmed that the biochemical test, which is the determination of metalloproteinases in tissues, is a useful diagnostic tool to differentiate inflammatory from non-inflammatory tissues.
炎症性肠病之一是克罗恩病。尽管自1932年以来这个术语就在医学界使用，但在20世纪末和21世纪初出版物数量显著增加。克罗恩病是一种无法完全治愈的疾病。在许多情况下，它是慢性的，即会复发。医生采取的所有预防和治疗措施旨在抑制疾病发展，并将疾病发展所产生的任何潜在“副作用”的发生率降至最低。诊断方法之一是对炎症组织和血液中的金属蛋白酶进行定性和定量测定。该研究的目的是对诊断为克罗恩病的患者的炎症性肠组织中的金属蛋白酶进行定量和定性测定。体外研究是对诊断为克罗恩病的患者的手术组织进行的。结果表明，与取自无炎症迹象（定义为健康）的切除边缘组织相比，炎症组织中金属蛋白酶-3、-7、-8、-9的浓度更高。实验证实，在组织中测定金属蛋白酶的生化检测是区分炎症组织和非炎症组织的有用诊断工具。
