十二烷基硫酸钠-聚丙烯酰胺凝胶电泳后用溴化乙锭染色检测脂多糖。
Detection of lipopolysaccharides by ethidium bromide staining after sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
作者信息
Kido N, Ohta M, Kato N
机构信息
Department of Bacteriology, Nagoya University School of Medicine, Aichi, Japan.
出版信息
J Bacteriol. 1990 Feb;172(2):1145-7. doi: 10.1128/jb.172.2.1145-1147.1990.
Abstract
A rapid and easy method for staining lipopolysaccharides with ethidium bromide is described. Lipopolysaccharides could be visualized by ethidium bromide with almost the same sensitivity as found with the silver-staining method in less than 30 min. The ethidium bromide-staining method was particularly suitable for staining lipopolysaccharides possessing acidic O-specific polysaccharides, which were poorly visualized by silver staining.
摘要
本文描述了一种用溴化乙锭染色脂多糖的快速简便方法。在不到30分钟的时间内，溴化乙锭就能使脂多糖显色，其灵敏度与银染法几乎相同。溴化乙锭染色法特别适用于对含有酸性O-特异性多糖的脂多糖进行染色，而银染法对这类脂多糖的显色效果较差。
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