通过单克隆抗体鉴定辛德毕斯病毒糖蛋白复合物中的新表位
Identification by monoclonal antibodies of a new epitope in the glycoprotein complex of Sindbis virus.
作者信息
Righi M, Radaelli A, Castagnoli P R, Liboi E, De Giuli Morghen C
出版信息
J Virol Methods. 1983 Apr;6(4):203-14. doi: 10.1016/0166-0934(83)90047-2.
Monoclonal antibodies against a deletion mutant of Sindbis virus were produced and characterized in order to determine the fine mapping and functional activities of single viral epitopes. All monoclonal antibodies so far tested showed a certain degree of reciprocal competition and were directed against an antigenic determinant which was present only on the undissociated complex of the E1 and E2 glycoproteins. A biological assay measuring viral haemagglutination showed no decrease in the titre of viral samples preincubated with monoclonal antibodies. Conversely, a reduction in viral infectivity was demonstrated, particularly with two of these antibodies. The results suggest that the antibodies which we characterized seem to recognize a new epitope which is represented on both glycoproteins on the surface of this mutant of Sindbis virus.
为了确定单个病毒表位的精细定位和功能活性，制备并鉴定了针对辛德毕斯病毒缺失突变体的单克隆抗体。到目前为止测试的所有单克隆抗体都表现出一定程度的相互竞争，并且针对的是仅存在于E1和E2糖蛋白未解离复合物上的抗原决定簇。一项测量病毒血凝的生物学试验表明，与单克隆抗体预孵育的病毒样本的滴度没有降低。相反，证明病毒感染性降低，特别是其中两种抗体。结果表明，我们鉴定的抗体似乎识别了一个新表位，该表位存在于辛德毕斯病毒这个突变体表面的两种糖蛋白上。
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