Mittal K R, Higgins R, Lariviere S
J Clin Microbiol. 1983 May;17(5):787-90. doi: 10.1128/jcm.17.5.787-790.1983.
Serological properties of antigens extracted from strains of Haemophilus pleuropneumoniae belonging to seven different serotypes were investigated. Antisera were prepared in rabbits against Formalin-treated whole cell suspensions as well as autoclaved cell suspensions. Saline and heat extracts and their alcohol precipitate antigens of H. pleuropneumoniae were used in the indirect hemagglutination test. All the antigens used were easily adsorbed directly onto sheep erythrocytes. Saline extract antigen showed maximum type specificity. Heating of the whole cell suspension revealed the cross-reactive minor antigenic determinants. Thus, the heat extract preparations had both type-specific and species-specific antigens. It is suggested that the indirect hemagglutination test may be useful for both serotyping and serodiagnosis of H. pleuropneumoniae infections in pigs.
对从七种不同血清型的胸膜肺炎放线杆菌菌株中提取的抗原的血清学特性进行了研究。用兔制备了针对经福尔马林处理的全细胞悬液以及经高压灭菌的细胞悬液的抗血清。胸膜肺炎放线杆菌的盐水提取物、热提取物及其乙醇沉淀抗原用于间接血凝试验。所有使用的抗原都很容易直接吸附到绵羊红细胞上。盐水提取物抗原表现出最大的型特异性。全细胞悬液加热后显示出交叉反应性次要抗原决定簇。因此，热提取物制剂具有型特异性和种特异性抗原。建议间接血凝试验可能对猪胸膜肺炎放线杆菌感染的血清分型和血清诊断都有用。
