Taichman L B, Reilly S S, LaPorta R F
J Invest Dermatol. 1983 Jul;81(1 Suppl):137s-40s. doi: 10.1111/1523-1747.ep12540909.
We have examined the growth of three epitheliotropic viruses in cultures of human epidermal keratinocytes: herpes simplex virus (HSV) type 1, adenovirus type 2 (Ad-2), and human papillomavirus (HPV) type 1. Differences were noted in the level of expression of each virus, and these differences may be related to a dependency or lack of dependency on keratinocyte differentiation for complete viral growth. Of the three viruses studied, HSV was the only one to replicate productively in all cells of the culture. Its expression was independent of keratinocyte differentiation. This is unlike Ad-2, which infected all cells in the culture but replicated productively only in the suprabasal cells. Basal keratinocytes were shown to be infected, but for unknown reasons, they appeared in most instances to be nonpermissive for Ad-2 replication. Infected basal keratinocytes became permissive when they reached a suprabasal position. Ad-2 appears to require keratinocyte differentiation for full expression in culture. Following infection with HPV, cultured keratinocytes showed no evidence of productive replication. However, 50 to 250 copies of HPV DNA could be detected in each cell (average) as stable nonintegrated molecules. Viral DNA replication has been shown to occur in the younger cells and not in the older, more differentiated keratinocytes. The failure of HPV to be fully expressed in culture may be related, in part, to incomplete differentiation of the keratinocyte in vitro. The major conclusions of this study are (1) that keratinocyte differentiation is likely to play a role in the expression of some epitheliotropic viruses in culture, and (2) that keratinocyte differentiation may be a factor in the pathogenesis of certain viral diseases of keratinizing epithelia.
1型单纯疱疹病毒（HSV）、2型腺病毒（Ad-2）和1型人乳头瘤病毒（HPV）。每种病毒的表达水平存在差异，这些差异可能与病毒完全生长对角质形成细胞分化的依赖性或非依赖性有关。在所研究的三种病毒中，HSV是唯一能在培养物的所有细胞中高效复制的病毒。其表达不依赖于角质形成细胞的分化。这与Ad-2不同，Ad-2能感染培养物中的所有细胞，但仅在上皮基底上层细胞中高效复制。基底角质形成细胞虽被证明受到感染，但由于未知原因，在大多数情况下它们似乎对Ad-2复制不敏感。受感染的基底角质形成细胞到达上皮基底上层位置后就变得敏感。Ad-2似乎需要角质形成细胞分化才能在培养物中充分表达。HPV感染后，培养的角质形成细胞未显示出高效复制的迹象。然而，每个细胞（平均）中可检测到50至250个HPV DNA拷贝，它们以稳定的非整合分子形式存在。已证明病毒DNA复制发生在较年轻的细胞中，而不是在较老、分化程度更高的角质形成细胞中。HPV在培养物中未能充分表达可能部分与体外角质形成细胞分化不完全有关。本研究的主要结论是：（1）角质形成细胞分化可能在某些亲上皮性病毒在培养物中的表达中起作用；（2）角质形成细胞分化可能是某些角化上皮病毒性疾病发病机制中的一个因素。
