Crum C P, Nuovo G, Friedman D, Silverstein S J
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
J Virol. 1988 Jan;62(1):84-90. doi: 10.1128/JVI.62.1.84-90.1988.
The accumulation of human papillomavirus type 16 (HPV-16)-specific RNAs in tissue sections from biopsies of patients with genital precancers was studied by in situ hybridization with single-stranded 35S-labeled RNA. These analyses revealed that the most abundant early-region RNAs were derived from the E4 and E5 open reading frames (ORFs). RNAs homologous to the E6/E7 ORFs were also detected, whereas RNAs homologous to the intervening E1 ORF were not. This suggests that the E4 and E5 mRNAs are derived by splicing to the upstream E6/E7 ORFs, consistent with studies of HPV-11 in condylomata (L. T. Chow et al., Cancer Cells (Cold Spring Harbor) 5:55-72, 1987). Abundant RNAs homologous to the 5' portion of L1 were also detected. These RNAs were localized to the apical strata of the epithelium. HPV-16 RNAs accumulated in discrete regions of these lesions, and when present were most abundant in the upper cell layers of the precancerous epithelium. RNAs homologous to early ORFs were also detected in some germinal cells within the basal layer of the epithelium.
通过用单链35S标记的RNA进行原位杂交，研究了16型人乳头瘤病毒（HPV-16）特异性RNA在生殖器癌前病变患者活检组织切片中的积累情况。这些分析表明，最丰富的早期区域RNA来自E4和E5开放阅读框（ORF）。也检测到了与E6/E7 ORF同源的RNA，而未检测到与中间的E1 ORF同源的RNA。这表明E4和E5 mRNA是通过剪接至上游的E6/E7 ORF产生的，这与尖锐湿疣中HPV-11的研究结果一致（L.T. Chow等人，《癌细胞》（冷泉港）5:55-72，1987年）。还检测到了与L1 5'部分同源的丰富RNA。这些RNA定位于上皮的顶端层。HPV-16 RNA在这些病变的离散区域积累，并且在癌前上皮的上层细胞中存在时最为丰富。在基底细胞层的一些生发细胞中也检测到了与早期ORF同源的RNA。
