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[不同条件下血小板上HL-A抗原的显示]

[The demonstration of HL-A antigens on thrombocytes under various conditions].

作者信息

Bube F W, Seegers D, Siebel E, Heumann H

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(2):240-7.

PMID:61918
Abstract

Platelets of healthy test persons were gained according to the method of Aster (avital cells) and according to the procedure of BUBE and GMURZYNSKI (vital cells). Because of their tendency towards aggregation vital cells do bind any HL-A antibodies and whereas avital cells will fix antibodies and complement depending on the height of the titre. Thrombocytes stored in the own serum will respond less to the micro-C'-fixation test at + 4 degrees C as well as--196 degrees C, whereas storage in NaN3/NaCl and under the conditions of the own serum will reveal no differences up to--196 degrees C in each case.

摘要

健康测试者的血小板是按照阿斯特(无活力细胞)的方法以及布贝和古穆尔津斯基(有活力细胞)的程序获取的。由于有活力细胞有聚集的倾向,它们不会结合任何HL - A抗体,而无活力细胞会根据滴度的高低固定抗体和补体。储存在自身血清中的血小板在 + 4摄氏度以及 - 196摄氏度时对微量C'固定试验的反应较小,而储存在NaN3/NaCl中以及在自身血清条件下,在每种情况下直到 - 196摄氏度都不会显示出差异。

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