Sjölund K, Schaffalitzky O B, Muckadell D E, Fahrenkrug J, Håkanson R, Peterson B G, Sundler F
Gut. 1983 Aug;24(8):724-33. doi: 10.1136/gut.24.8.724.
Neurones containing VIP, substance P, or enkephalin were studied by immunocytochemistry in intestinal specimens from 27 patients with Crohn's disease. Also several endocrine cell systems in the gut were examined. The results were compared with those from a control group of 26 patients. The relative frequency of various endocrine cells did not differ overtly from that in controls. Vasoactive intestinal polypeptide and substance P nerve fibres were distributed in all layers of the gut wall, including the submucosal and myenteric plexuses, whereas enkephalin fibres were restricted to the smooth muscle layer and the myenteric plexus. The distribution and frequency of the peptide-containing nerve fibres were the same in Crohn's disease patients as in control patients. A proportion of these nerve fibres, however, were notably coarse in the Crohn's disease patients. This was particularly apparent in the afflicted parts of the intestine although it was noted also in non-afflicted parts. The concentration of VIP and substance P (expressed as pmol/g wet weight) did not, however, exceed that of the control group.
采用免疫细胞化学方法，对27例克罗恩病患者的肠道标本中含血管活性肠肽（VIP）、P物质或脑啡肽的神经元进行了研究。同时，对肠道中的几种内分泌细胞系统进行了检测。将结果与26例对照组患者的结果进行了比较。各种内分泌细胞的相对频率与对照组相比无明显差异。血管活性肠肽和P物质神经纤维分布于肠壁各层，包括黏膜下丛和肌间神经丛，而脑啡肽纤维仅限于平滑肌层和肌间神经丛。含肽神经纤维的分布和频率在克罗恩病患者和对照患者中相同。然而，在克罗恩病患者中，这些神经纤维中有一部分明显增粗。这在肠道病变部位尤为明显，不过在未受累部位也有发现。然而，VIP和P物质的浓度（以每克湿重的皮摩尔数表示）并未超过对照组。
