Wolf W A, Kuhn D M
J Chromatogr. 1983 Jun 10;275(1):1-9. doi: 10.1016/s0378-4347(00)84338-8.
L-Tryptophan, 5-hydroxytryptophan (5-HTP), 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid (5-HIAA) from discrete regions of brain can be resolved by isocratic elution on a C-18 reversed-phase column and quantitated by native fluorescence measurement. This method is rapid and simple and can reproduceably detect 5-hydroxytryptamine, tryptophan and 5-HIAA with picogram sensitivity. The detection of 5-HTP in brain extracts from untreated animals is difficult because of the extremely low endogenous levels of 5-HTP. Quantitation of 5-HTP presents no problem in animals injected with 5-HTP or with an inhibitor of L-aromatic amino acid decarboxylase. Brain tissue requires minimal treatment for assay and the hydroxyindoles are stable enough to allow automated processing.
来自大脑不同区域的L-色氨酸、5-羟色氨酸（5-HTP）、5-羟色胺和5-羟吲哚乙酸（5-HIAA），可通过在C-18反相柱上进行等度洗脱来分离，并通过天然荧光测量进行定量。该方法快速简便，能够以皮克灵敏度可重复地检测5-羟色胺、色氨酸和5-HIAA。由于5-HTP的内源性水平极低，在未经处理的动物脑提取物中检测5-HTP很困难。在注射了5-HTP或L-芳香族氨基酸脱羧酶抑制剂的动物中，5-HTP的定量分析不存在问题。脑组织用于检测所需的处理极少，且羟吲哚足够稳定，可进行自动化处理。
