Lane H C, Whalen G, Fauci A S
J Clin Invest. 1983 Aug;72(2):636-47. doi: 10.1172/JCI111013.
We have recently noted marked differences between the in vitro responses of human B lymphocytes to stimulation with soluble antigens vs. stimulation with mitogens. In the present study, these differences were analyzed in terms of the precursor frequencies for the T cells and B cells involved and in terms of the radiation sensitivity of the T cells providing help in the two systems. Marked differences were found between antigen-induced and mitogen-induced systems with regard to T cell precursor frequencies and radiation sensitivity. In contrast, the precursor frequencies for the B cells involved in the two systems were approximately the same. In addition, having developed a system for the study of human antigen-specific B cell responses, we were interested in delineating the nature of the allogeneic effects that might be operative in this system. Marked allogeneic effects, both positive and negative, were noted in this system and will need to be taken into account in any studies that try to address the question of the genetic restriction, if any, that exists in human antigen-specific T cell-B cell collaboration. Appreciation of the marked differences between the antigen-specific and mitogen-induced activation and immunoregulation of human B cell responses will be of importance in understanding the relationship between specificity and nonspecificity of antibody production in normal and disease states.
我们最近注意到，人类B淋巴细胞对可溶性抗原刺激的体外反应与对有丝分裂原刺激的体外反应之间存在显著差异。在本研究中，从参与其中的T细胞和B细胞的前体频率以及在这两种系统中提供辅助的T细胞的辐射敏感性方面分析了这些差异。在抗原诱导系统和有丝分裂原诱导系统之间，在T细胞前体频率和辐射敏感性方面发现了显著差异。相比之下，参与这两种系统的B细胞的前体频率大致相同。此外，在开发了一种用于研究人类抗原特异性B细胞反应的系统后，我们有兴趣描述可能在该系统中起作用的同种异体效应的性质。在该系统中观察到了显著的同种异体效应，包括阳性和阴性效应，在任何试图解决人类抗原特异性T细胞 - B细胞协作中是否存在遗传限制问题的研究中都需要考虑这些效应。认识到人类B细胞反应的抗原特异性和有丝分裂原诱导的激活及免疫调节之间的显著差异，对于理解正常和疾病状态下抗体产生的特异性与非特异性之间的关系至关重要。
