Troye-Blomberg M, Perlmann H, Patarroyo M E, Perlmann P
Clin Exp Immunol. 1983 Aug;53(2):345-53.
The antigen-induced DNA synthesis in vitro in lymphocytes from patients with acute Plasmodium falciparum malaria was investigated. The patients and healthy controls from Sweden or Colombia were the same as those studied in the accompanying paper (Troye-Blomberg et al., 1983). The malarial antigens used were sonicated membrane preparations or purified and concentrated supernatants from in vitro cultures of P. falciparum; similar preparations derived from normal human erythrocytes served as control antigen. In the patients' lymphocytes P. falciparum antigens induced a weak or moderate but significant stimulation of DNA synthesis, peaking after 3-4 days of incubation. This early response was specific for P. falciparum since it was not obtained with lymphocytes from healthy donors nor with those from patients with acute P. vivax or P. ovale malaria. No antigen-induced response was seen in about half of the P. falciparum patients. However in a few negative cases, available for consecutive testing, positive reactions were seen with lymphocytes taken 2 weeks after infection when the blood of these patients was free of parasites. The early response induced in patients' lymphocytes to the P. falciparum antigens was not obtained with RBC antigen. However, these preparations frequently induced a response rising to significant levels later during incubation (day 5-6). Similar delayed responses were obtained when either patients' or control donors' lymphocytes were exposed to the P. falciparum antigens. This indicates that both the RBC and the parasite preparations contained mitogenic substances affecting human lymphocytes in general and easily obscuring the P. falciparum specific response seen only in the patients. This latter response was relatively low and short lived, suggesting that it reflected a secondary in vitro stimulation of in vivo primed lymphocytes and that it was regulated by suppressor mechanisms.
对急性恶性疟原虫疟疾患者淋巴细胞的体外抗原诱导DNA合成进行了研究。患者以及来自瑞典或哥伦比亚的健康对照与随附论文（Troye-Blomberg等人，1983年）中所研究的相同。所用的疟疾抗原是经超声处理的膜制剂或来自恶性疟原虫体外培养物的纯化浓缩上清液；来自正常人红细胞的类似制剂用作对照抗原。在患者淋巴细胞中，恶性疟原虫抗原诱导了DNA合成的微弱或中等但显著的刺激，在孵育3 - 4天后达到峰值。这种早期反应对恶性疟原虫具有特异性，因为健康供体的淋巴细胞以及间日疟或卵形疟急性患者的淋巴细胞均未出现这种反应。约一半的恶性疟患者未观察到抗原诱导的反应。然而，在少数可进行连续检测的阴性病例中，当这些患者的血液中无寄生虫时，在感染后2周采集的淋巴细胞中观察到了阳性反应。患者淋巴细胞对恶性疟原虫抗原诱导的早期反应在红细胞抗原刺激下未出现。然而，这些制剂在孵育后期（第5 - 6天）经常诱导反应上升至显著水平。当患者或对照供体的淋巴细胞暴露于恶性疟原虫抗原时，也获得了类似的延迟反应。这表明红细胞和寄生虫制剂均含有影响人类淋巴细胞的促有丝分裂物质，通常容易掩盖仅在患者中观察到的恶性疟原虫特异性反应。后一种反应相对较低且持续时间短，表明它反映了体内致敏淋巴细胞的体外二次刺激，并且受抑制机制调节。
