Tsurufuji M, Benacerraf B, Sy M S
J Exp Med. 1983 Sep 1;158(3):932-45. doi: 10.1084/jem.158.3.932.
Suppressor T cells (Ts-1) induced with trinitrophenyl (TNP)-conjugated syngeneic spleen cells (TNP-SC) can be enriched on antigen-coated plates and are afferent suppressors. In addition, these suppressor cells produced soluble suppressor factors (TsF) that were active in vivo. Therefore, the Ts-1 cells in the TNP system are very similar to the Ts-1 cells in other systems we have studied earlier. Further characterization of these TsF-1 revealed that TsF-1 obtained from TNP-SC-induced Ts-1 is major histocompatibility complex restricted in its activity. Injection of TNP-specific TsF-1 into naive mice did not induce Ts-2 unless additional corresponding antigen was provided. Moreover, the Ts-2 cells induced by administration of both TsF-1 and trinitrobenzene sulfonic acid were antigen specific rather than antiidiotypic.
用三硝基苯基(TNP)偶联的同基因脾细胞(TNP-SC)诱导产生的抑制性T细胞(Ts-1)可在抗原包被的平板上富集,且为传入性抑制细胞。此外,这些抑制细胞产生的可溶性抑制因子(TsF)在体内具有活性。因此,TNP系统中的Ts-1细胞与我们之前研究的其他系统中的Ts-1细胞非常相似。对这些TsF-1的进一步表征显示,从TNP-SC诱导的Ts-1获得的TsF-1其活性受主要组织相容性复合体限制。将TNP特异性TsF-1注射到未接触过抗原的小鼠体内,除非提供额外的相应抗原,否则不会诱导产生Ts-2。此外,通过同时给予TsF-1和三硝基苯磺酸诱导产生的Ts-2细胞是抗原特异性的,而非抗独特型的。