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从鼠伤寒沙门氏菌LT2的双庚糖粗糙突变体中分离的转运RNA在小鼠伤寒感染中的免疫原性。

Immunogenicity of transfer RNA isolated from a two-heptose rough mutant of Salmonella typhimurium LT2 in mouse typhoid infection.

作者信息

Kita E, Kashiba S

出版信息

Immunology. 1983 Nov;50(3):369-76.

PMID:6195095
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1454259/
Abstract

Transfer ribonucleic acid (tRNA) was isolated from a two-heptose mutant of Salmonella typhimurium LT2 (strain SL1004) and was found to afford 100% mouse protection against challenge with 1000 LD50 of strain LT2. The intraperitoneal minimum effective dose of tRNA was 5 micrograms RNA per mouse and this dose was significantly lower than that of ribosomal RNA for ddY mouse strain. The protective immunity was independent of the presence of antibodies to cell-surface antigens, and was transferred mainly by T cells. The protective moiety of tRNA was sensitive to ribonuclease digestion which resulted in 85% reduction in the mouse survival rate, but was completely resistant to protease digestion. The present study demonstrates that the immunogenic activity of salmonella RNA is present in both ribosomal RNA and tRNA.

摘要

从鼠伤寒沙门氏菌LT2的双庚糖突变体（菌株SL1004）中分离出转移核糖核酸（tRNA），发现其能为小鼠提供100%的保护，使其免受1000个LT2菌株半数致死量的攻击。tRNA腹腔注射的最小有效剂量为每只小鼠5微克RNA，该剂量显著低于ddY小鼠品系的核糖体RNA剂量。保护性免疫与细胞表面抗原抗体的存在无关，主要由T细胞介导。tRNA的保护部分对核糖核酸酶消化敏感，这导致小鼠存活率降低85%，但对蛋白酶消化完全抗性。本研究表明，沙门氏菌RNA的免疫原活性存在于核糖体RNA和tRNA中。

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Immunogenicity of transfer RNA isolated from a two-heptose rough mutant of Salmonella typhimurium LT2 in mouse typhoid infection.从鼠伤寒沙门氏菌LT2的双庚糖粗糙突变体中分离的转运RNA在小鼠伤寒感染中的免疫原性。
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本文引用的文献

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Antibody-producing cell responses to an isolated outer membrane protein and to complexes of this antigen with lipopolysaccharide or with vesicles of phospholipids from Proteus mirabilis.针对奇异变形杆菌一种分离的外膜蛋白以及该抗原与脂多糖或磷脂囊泡形成的复合物产生抗体的细胞反应。
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