Kurtz A B, Gray R S, Markanday S, Nabarro J D
Diabetologia. 1983 Oct;25(4):322-4. doi: 10.1007/BF00253194.
Sera from patients with different types of protamine-insulin were assayed for IgG antibody to protamine. A high prevalence of circulating antibody was found in patients treated with either bovine isophane insulin (26 out of 28 patients; 26 of whom also had antibodies to insulin), or bovine protamine zinc insulin (27 out of 30 patients; all 30 had antibodies to insulin). In sera from 24 patients treated with highly purified porcine isophane insulin, protamine antibody was detected in nine; circulating insulin-antibody was detected in 12 patients, eight of whom had protamine-antibody; in the 12 patients with no detectable antibody to insulin, antibody to protamine was detected in only one (x2 = 8.7, p less than 0.01). This relationship between insulin and protamine antigenicity is of interest as it suggests that the protamine-insulin complex is itself immunogenic.
对不同类型鱼精蛋白胰岛素患者的血清进行鱼精蛋白IgG抗体检测。接受牛低精蛋白胰岛素治疗的患者（28例中有26例；其中26例也有胰岛素抗体）或牛精蛋白锌胰岛素治疗的患者（30例中有27例；所有30例均有胰岛素抗体）中，循环抗体的患病率较高。在接受高纯度猪低精蛋白胰岛素治疗的24例患者的血清中，9例检测到鱼精蛋白抗体；12例患者检测到循环胰岛素抗体，其中8例有鱼精蛋白抗体；在12例未检测到胰岛素抗体的患者中，仅1例检测到鱼精蛋白抗体（χ2 = 8.7，p<0.01）。胰岛素和鱼精蛋白抗原性之间的这种关系很有意思，因为它表明鱼精蛋白胰岛素复合物本身具有免疫原性。
