Samuel T, Kolk A H, Rümke P
Clin Exp Immunol. 1978 Aug;33(2):252-60.
A complement fixation study with human, monkey and rabbit sera, using purified sperm nuclear basic proteins as antigens, led to the following conclusions. (1) Protamines, the sperm-specific basic nuclear proteins, may be immunogenic in mammalians. (2) Antibodies detected in the indirect immunofluorescence test on human swollen sperm heads in sera from infertile and vasectomized men, are directed primarily against human protamines. (3) The results obtained suggested that differences in the immunization site and/or in the configuration of the immunizing protamine, may lead to the formation of antibodies directed against different antigenic determinants. Autoimmunity to protamines, following vasectomy or in infertile men, is accompanied by the formation of antibodies cross-reacting with common antigenic determinants present in protamines of other species. Induction of immunity to protamines by means of immunization with protamines-RNA complexes (in rabbits), or protamine-insulin complexes (in humans), leads to the formation of antibodies reacting more specifically with the immunizing protamine, showing only slight cross-reaction with other protamines. (4) The histone-like fraction present in mature human spermatozoa is composed mainly of histone fraction H2B.
一项以纯化的精子核碱性蛋白为抗原，用人、猴和兔血清进行的补体结合研究得出了以下结论。（1）鱼精蛋白，即精子特异性的核碱性蛋白，在哺乳动物中可能具有免疫原性。（2）在不育和输精管切除男性血清中对人肿胀精子头部进行的间接免疫荧光试验中检测到的抗体，主要针对人鱼精蛋白。（3）所获得的结果表明，免疫部位和/或免疫鱼精蛋白的构型差异，可能导致针对不同抗原决定簇的抗体形成。输精管切除后或不育男性中对鱼精蛋白的自身免疫，伴随着与其他物种鱼精蛋白中存在的共同抗原决定簇发生交叉反应的抗体形成。通过用鱼精蛋白-RNA复合物（在兔中）或鱼精蛋白-胰岛素复合物（在人中）免疫诱导对鱼精蛋白的免疫，会导致形成与免疫鱼精蛋白反应更具特异性的抗体，与其他鱼精蛋白仅表现出轻微的交叉反应。（4）成熟人类精子中存在的组蛋白样部分主要由组蛋白H2B部分组成。
