Kabawat S E, Bast R C, Bhan A K, Welch W R, Knapp R C, Colvin R B
Int J Gynecol Pathol. 1983;2(3):275-85. doi: 10.1097/00004347-198303000-00005.
OC125, a murine monoclonal antibody, recognizes an antigenic determinant (CA125) that is associated with greater than 80% of epithelial ovarian neoplasms of serous, endometrioid, clear cell, and undifferentiated types. In the present report, a sensitive biotin-avidin immunoperoxidase technique was used to determine reactivity of OC125 with normal adult and fetal tissues, as well as with neoplasms of nonovarian origin. In fetal tissues, the antibody reacted with amnion and with derivatives of the coelomic epithelium, i.e., the müllerian epithelium and the lining cells of the peritoneum, pleura, and pericardium. Among adult tissues, OC125 reacted with the epithelium of fallopian tubes, endometrium, and endocervix. The CA125 determinant was also detected on mesothelial cells in the adult pleura, pericardium, and peritoneum, particularly in areas of inflammation and adhesion. Curiously, the surface epithelium of normal fetal and adult ovaries, thought to be derived from coelomic epithelium, did not express the determinant, except in inclusion cysts, areas of metaplasia, and papillary excrescences. Of neoplastic tissues of nonovarian origin, OC125 reacted consistently only with adenocarcinomas of the endocervix, endometrium, and fallopian tube, and with mesotheliomas. Only seven of 64 nongynecological tumors tested reacted with OC125. Thus, OC125 detects a differentiation antigen shared by fetal coelomic epithelium and its derivatives in the fetus and the adult. Apparently, this antigen disappears early in the course of formation of the ovarian epithelium and is reexpressed in certain reactive and neoplastic lesions, a process that could be termed "molecular metaplasia."
OC125是一种鼠单克隆抗体，可识别一种抗原决定簇（CA125），该抗原决定簇与80%以上的浆液性、子宫内膜样、透明细胞和未分化型上皮性卵巢肿瘤相关。在本报告中，使用了一种灵敏的生物素-抗生物素蛋白免疫过氧化物酶技术来测定OC125与正常成人及胎儿组织以及非卵巢来源肿瘤的反应性。在胎儿组织中，该抗体与羊膜以及体腔上皮的衍生物发生反应，即苗勒上皮以及腹膜、胸膜和心包的衬里细胞。在成人组织中，OC125与输卵管、子宫内膜和子宫颈内膜的上皮发生反应。在成人胸膜、心包和腹膜的间皮细胞上也检测到了CA125决定簇，特别是在炎症和粘连区域。奇怪的是，正常胎儿和成人卵巢的表面上皮，被认为源自体腔上皮，除了包涵囊肿、化生区域和乳头状赘生物外，不表达该决定簇。在非卵巢来源的肿瘤组织中，OC125仅与子宫颈、子宫内膜和输卵管的腺癌以及间皮瘤始终发生反应。在检测的64例非妇科肿瘤中，只有7例与OC125发生反应。因此，OC125检测到胎儿和成人中胎儿体腔上皮及其衍生物共有的一种分化抗原。显然，这种抗原在卵巢上皮形成过程的早期消失，并在某些反应性和肿瘤性病变中重新表达，这一过程可称为“分子化生”。
