Bartholomew J S, Anderson J C
Histochem J. 1983 Oct;15(10):941-51. doi: 10.1007/BF01002490.
The distribution of hyaluronic acid and proteoglycans in bovine thoracic aorta was studied by Alcian Blue staining of frozen tissue sections under controlled electrolyte conditions with and without prior enzymic digestion. Some sections were digested with chondroitinase ABC, testicular hyaluronidase or bacterial collagenase and subsequent staining permitted conclusions to be drawn about the distribution of specific glycosaminoglycans within the tissue. The total glycosaminoglycan content was maximal in the intima and decreased across the arterial wall to the outermost adventitial layer. The content of proteoglycan containing chondroitin sulphate and/or dermatan sulphate chains paralleled this distribution. However, other glycosaminoglycans also contributed significantly to staining, although there was no evidence for any appreciable concentration of heparin or highly sulphated heparan sulphate. Several experiments indicated that proteoglycan containing chondroitin sulphate and/or dermatan sulphate was associated with elastic laminae which were often seen stained along their periphery. Hyaluronic acid was present at significant concentrations in all locations of the aorta and there was evidence for a similar distribution of heparan sulphate which was possible also present at a high concentration in the endothelium. Staining of sections after treatment with 4 M guanidinium chloride confirmed that this extractant removed most of the proteoglycan from the tissue section.
在可控电解质条件下，对经酶消化和未经酶消化的牛胸主动脉冷冻组织切片进行阿尔辛蓝染色，研究透明质酸和蛋白聚糖的分布。部分切片用软骨素酶ABC、睾丸透明质酸酶或细菌胶原酶消化，随后的染色有助于推断特定糖胺聚糖在组织内的分布情况。总糖胺聚糖含量在内膜中最高，沿动脉壁向外膜最外层逐渐降低。含硫酸软骨素和/或硫酸皮肤素链的蛋白聚糖含量呈类似分布。然而，其他糖胺聚糖对染色也有显著贡献，尽管没有证据表明存在任何可观浓度的肝素或高度硫酸化的硫酸乙酰肝素。多项实验表明，含硫酸软骨素和/或硫酸皮肤素的蛋白聚糖与弹性膜相关，弹性膜周边常可见染色。透明质酸在主动脉的所有部位均有显著浓度，有证据表明硫酸乙酰肝素分布相似，其在内皮中也可能以高浓度存在。用4M氯化胍处理后的切片染色证实，这种萃取剂从组织切片中去除了大部分蛋白聚糖。
