Heggeness M H, Simon M, Singer S J
Proc Natl Acad Sci U S A. 1978 Aug;75(8):3863-6. doi: 10.1073/pnas.75.8.3863.
By indirect immunofluorescence techniques, microtubules and mitochondria were localized in normal rat kidney cells, human WI38 fibroblasts, mouse peritoneal macrophages, and a putative smooth muscle rat cell line, in monolayer culture. The mitochondria were found to be arranged along the cytoplasmic microtubules in each cell type. Disruption of the microtubules with colcemid caused a redistribution of the mitochondria in these cells. There was no correlation between the location of the mitochondria and actin-containing filaments. This evidence suggests that mitochondria are directly or indirectly associated with microtubules in these cells.
通过间接免疫荧光技术，在单层培养的正常大鼠肾细胞、人WI38成纤维细胞、小鼠腹腔巨噬细胞和一种假定的大鼠平滑肌细胞系中，对微管和线粒体进行了定位。发现线粒体在每种细胞类型中都沿着细胞质微管排列。用秋水仙酰胺破坏微管会导致这些细胞中线粒体的重新分布。线粒体的位置与含肌动蛋白的细丝之间没有相关性。这一证据表明，在这些细胞中，线粒体直接或间接地与微管相关联。
