Takei I, Sumida T, Taniguchi M
J Exp Med. 1983 Dec 1;158(6):1912-23. doi: 10.1084/jem.158.6.1912.
An acceptor hybridoma with a receptor that recognizes the keyhole limpet hemocyanin (KLH)-specific suppressor T cell factor (KLH-TsF) was established after the fusion of C57BL/6 splenic T cells enriched with KLH-coated petri dishes. The cloned hybridoma (34S-281) could be specifically activated by stimulation with the conventional KLH-TsF or monoclonal KLH-TsF from three different hybridomas in the absence of the relevant antigen (KLH) and it started to produce another factor that suppresses the antibody response against DNP-KLH in a KLH-specific fashion. The KLH specificity of the TsF was required for activation. The new factor was found not to bind the KLH but to be absorbed with the KLH-TsF-producing hybridoma. It is thus strongly suggested that the acceptor site has a complementary structure (antiidiotype) for the KLH-TsF. Moreover, the idiotypic determinant on KLH-TsF was found to have a structure similar to that on some of the anti-KLH antibodies, since the acceptor hybridoma was specifically killed by the conventional anti-KLH antibodies and complement. Drawing on the above results, the idiotype-antiidiotype network in the conventional antigen system is discussed.
在用包被钥孔戚血蓝蛋白(KLH)的培养皿富集C57BL/6脾T细胞后,融合得到了一种具有识别KLH特异性抑制性T细胞因子(KLH-TsF)受体的受体杂交瘤。克隆的杂交瘤(34S-281)在无相关抗原(KLH)存在的情况下,可被传统的KLH-TsF或来自三种不同杂交瘤的单克隆KLH-TsF刺激而特异性激活,并开始产生另一种以KLH特异性方式抑制针对DNP-KLH抗体反应的因子。激活需要TsF的KLH特异性。发现新因子不与KLH结合,但可被产生KLH-TsF的杂交瘤吸收。因此,强烈提示受体位点具有与KLH-TsF互补的结构(抗独特型)。此外,发现KLH-TsF上的独特型决定簇具有与某些抗KLH抗体上的结构相似,因为受体杂交瘤被传统抗KLH抗体和补体特异性杀伤。根据上述结果,讨论了传统抗原系统中的独特型-抗独特型网络。