Snary D, Flint J E, Wood J N, Scott M T, Chapman M D, Dodd J, Jessell T M, Miles M A
Clin Exp Immunol. 1983 Dec;54(3):617-24.
Of 26 hybridomas secreting anti-Trypanosoma cruzi antibodies, two reacted with murine brain and spinal cord extracts but not other tissues. Both antibodies (5H7 and 3H3) had identical isotypes (IgM) and specificities as judged by western blotting. Antigens of molecular weight 58,000 and 37,000 in mouse brain and spinal cord, and 58,000 and 35,000 in T. cruzi were detected. Different tissue antigens were recognized by a previously reported T. cruzi mammalian neural tissue cross-reacting monoclonal antibody CE5; on immunofluorescence 5H7 stained some glia, and some central and peripheral neurones in rat tissue sections. Pooled sera from mice chronically infected with T. cruzi competed with binding of 5H7 to T. cruzi antigens.
在26株分泌抗克氏锥虫抗体的杂交瘤中，有两株与小鼠脑和脊髓提取物发生反应，但不与其他组织反应。通过蛋白质印迹法判断，两种抗体（5H7和3H3）具有相同的免疫球蛋白类型（IgM）和特异性。在小鼠脑和脊髓中检测到分子量为58,000和37,000的抗原，在克氏锥虫中检测到分子量为58,000和35,000的抗原。先前报道的克氏锥虫哺乳动物神经组织交叉反应单克隆抗体CE5识别不同的组织抗原；在免疫荧光实验中，5H7可使大鼠组织切片中的一些神经胶质细胞以及一些中枢和外周神经元染色。慢性感染克氏锥虫的小鼠的混合血清可与5H7与克氏锥虫抗原的结合发生竞争。
