Tibayrenc M, Miles M A
Trans R Soc Trop Med Hyg. 1983;77(1):76-83. doi: 10.1016/0035-9203(83)90021-4.
The enzyme profiles of three major Brazilian Trypanosoma cruzi zymodemes (Brazilian Z1, Brazilian Z2, Brazilian Z3) and of two principal Bolivian zymodemes were compared. Relationships were assessed both intuitively and by calculating genetic distances. One of the Bolivian zymodemes, Bolivian Z1, was closely related to the Brazilian Z1. The second Bolivian zymodeme, Bolivian Z2, was related to brazilian Z2 but differed from all the Brazilian zymodemes in the occurrence of typical heterozygous isozyme patterns in five out of 12 enzyme loci. Parental stocks and clones of Bolivian Z2 had the same putative heterozygous patterns. The evidence from enzyme profiles on the ploidy of T. cruzi and the possibility of recombination was considered. The presence of putative heterozygous patterns in Bolivian Z2 supported the hypothesis that T. cruzi is diploid. The definition of T. cruzi as a single polytypic species or as a species complex was considered to be dependent on the presence or absence of genetic recombination between or within the zymodemes, which has not been demonstrated in the ecotopes so far examined.
比较了巴西三种主要克氏锥虫酶谱型（巴西Z1、巴西Z2、巴西Z3）和玻利维亚两种主要酶谱型的酶谱。通过直观评估和计算遗传距离来评估它们之间的关系。玻利维亚的一种酶谱型，即玻利维亚Z1，与巴西Z1密切相关。玻利维亚的第二种酶谱型，即玻利维亚Z2，与巴西Z2相关，但在12个酶位点中的5个位点出现典型的杂合同工酶模式，这与所有巴西酶谱型不同。玻利维亚Z2的亲本菌株和克隆具有相同的假定杂合模式。考虑了来自酶谱的关于克氏锥虫倍性和重组可能性的证据。玻利维亚Z2中假定杂合模式的存在支持了克氏锥虫是二倍体的假设。克氏锥虫被定义为单一多型种还是种复合体，被认为取决于酶谱型之间或内部是否存在基因重组，而在目前所研究的生态环境中尚未证实这一点。
