Handley P S, Carter P L, Fielding J
J Bacteriol. 1984 Jan;157(1):64-72. doi: 10.1128/jb.157.1.64-72.1984.
Strains of Streptococcus salivarius were screened by negative staining for the presence of surface structures. Two structural subgroups were found, carrying either fibrils or fimbriae, projecting from the cell surface. Eight strains carried a very dense peritrichous array of fibrils of two distinct lengths. Long fibrils had an average length of 175 nm, and short fibrils had an average length of 95 nm. Two strains carried only long fibrils, one strain carried only short fibrils, and another strain carried a lateral tuft of very prominent fibrils of two lengths, with a fibrillar fuzz covering the remainder of the cell surface. In all the strains in which they were present, the long fibrils were unaffected by protease or trypsin treatment. In contrast, the short fibrils were completely digested by protease and partially removed by trypsin. Neither long nor short fibrils were affected structurally by mild pepsin digestion or by lipase. The Lancefield extraction procedure removed both long and short fibrils. These twelve fibrillar strains were therefore divisible into four structural subgroups. Extracts of all the fibrillar strains reacted with group K antiserum. The second main structural subgroup consisted of nine strains of S. salivarius, all of which carried morphologically identical, flexible fimbriae arranged peritrichously over the cell surface. The fimbriae were structurally distinct from fibrils and measured 0.5 to 1.0 micron long and 3 to 4 nm wide, with an irregular outline and no obvious substructure. There was no obvious reduction in the number of fimbriae after protease or trypsin treatment. Extracts of the fimbriated strains did not react with the group K antiserum. The two serological and structural subgroups could also be distinguished by colony morphology.
通过负染色法筛选唾液链球菌菌株，以检测其表面结构的存在情况。发现了两个结构亚组，它们分别带有从细胞表面伸出的纤丝或菌毛。八株菌株带有非常密集的周身纤丝阵列，纤丝有两种不同长度。长纤丝平均长度为175纳米，短纤丝平均长度为95纳米。两株菌株仅带有长纤丝，一株菌株仅带有短纤丝，另一株菌株带有一束非常突出的两种长度的侧生纤丝，细胞表面的其余部分覆盖着一层纤丝状绒毛。在所有存在长纤丝的菌株中，长纤丝不受蛋白酶或胰蛋白酶处理的影响。相比之下，短纤丝被蛋白酶完全消化，被胰蛋白酶部分去除。长纤丝和短纤丝在结构上均不受温和的胃蛋白酶消化或脂肪酶的影响。兰斯菲尔德提取程序去除了长纤丝和短纤丝。因此，这十二株纤丝菌株可分为四个结构亚组。所有纤丝菌株的提取物都与K组抗血清发生反应。第二个主要结构亚组由九株唾液链球菌组成，所有菌株都带有形态相同、柔韧的菌毛，周身排列在细胞表面。这些菌毛在结构上与纤丝不同，长度为0.5至1.0微米，宽度为3至4纳米，轮廓不规则，没有明显的亚结构。蛋白酶或胰蛋白酶处理后，菌毛数量没有明显减少。有菌毛菌株的提取物不与K组抗血清发生反应。这两个血清学和结构亚组也可以通过菌落形态来区分。
