Casewell M W, Desai N
J Hosp Infect. 1983 Dec;4(4):350-60. doi: 10.1016/0195-6701(83)90005-1.
The survival of various Gram-negative bacilli was evaluated by inoculating the finger-tips of volunteers and determining the number of recoverable organisms in finger washings taken at increasing time intervals up to 60 min. Three epidemic gentamicin-resistant multiply-resistant strains of Klebsiella aerogenes (capsular types K2, K16 and K21) survived better than Pseudomonas aeruginosa, Escherichia coli or non-epidemic antibiotic sensitive klebsiellae of corresponding capsular type. The survival of the epidemic klebsiellae was not altered by curing them of their resistance plasmids. Transfer of their plasmids to E. coli K12 and to sensitive non-epidemic strains of K. aerogenes of corresponding capsular type did not enhance the relatively poor survival of the recipients. We conclude that the enhanced survival on skin of multiply-resistant klebsiellae is not plasmid mediated but may well contribute to the transmissability of these organisms during hospital outbreaks especially when compared with E. coli and Ps. aeruginosa.
通过接种志愿者的指尖，并确定在长达60分钟的递增时间间隔采集的洗手样本中可培养出的微生物数量，来评估各种革兰氏阴性杆菌的存活情况。三株具有流行性庆大霉素耐药且多重耐药的产气克雷伯菌菌株（荚膜型别为K2、K16和K21）比铜绿假单胞菌、大肠杆菌或相应荚膜型别的非流行性抗生素敏感克雷伯菌存活得更好。去除流行性克雷伯菌的耐药质粒后，其存活情况并未改变。将它们的质粒转移至大肠杆菌K12以及相应荚膜型别的敏感非流行性产气克雷伯菌菌株，并未提高受体相对较差的存活率。我们得出结论，多重耐药克雷伯菌在皮肤上存活率的提高并非由质粒介导，但很可能有助于这些微生物在医院暴发期间的传播，尤其是与大肠杆菌和铜绿假单胞菌相比时。
