分子量测定程序。

Molecular weight determination program.

作者信息

Maina C V, Nolan G P, Szalay A A

出版信息

Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):695-702. doi: 10.1093/nar/12.1part2.695.

DOI:10.1093/nar/12.1part2.695

PMID:6198629

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321085/

Abstract

A computer program is described that will determine the molecular weight of DNA, RNA or protein molecules separated according to size by gel electrophoresis. It uses the sizes and migration distances of known molecules in a reference lane to compute a second or third order equation whose curve best fits the data points. It then computes the sizes of all molecules from this equation. Migration distances are measured and entered using an analog tablet. The program is written in Apple Pascal and designed to run on an Apple II Plus computer.

摘要

本文描述了一种计算机程序，它能根据凝胶电泳按大小分离出的DNA、RNA或蛋白质分子来确定其分子量。该程序利用参考泳道中已知分子的大小和迁移距离来计算二阶或三阶方程，其曲线能最佳拟合数据点。然后根据此方程计算所有分子的大小。迁移距离通过模拟数位板进行测量和输入。该程序用苹果Pascal语言编写，设计用于在苹果II Plus计算机上运行。