Beckmann E, Levitt D
Scand J Immunol. 1984 Jan;19(1):1-10. doi: 10.1111/j.1365-3083.1984.tb00894.x.
When peripheral blood lymphocytes (PBL) were stimulated in vitro with the rough form of type 2 Streptococcus pneumoniae R36a, the resulting plaque-forming cells (PFC) did not produce antibodies directed against phosphorylcholine, a major antigenic determinant of the cell wall C-polysaccharide. Instead, R36a stimulated polyclonal PFC in PBL and splenic lymphocytes. We compared the polyclonal responses stimulated by R36a with those induced by two well-characterized polyclonal activators (PA), Staphylococcus aureus Cowan I and pokeweed mitogen (PWM). We found that R36a was a poor mitogen for PBL, whereas the other two PA were potent mitogens; that the predominant isotype produced in response to all three PA was IgM; that adherent cells strongly inhibited the polyclonal PFC response to both R36a and Staph. aureus but not PWM; and that T cells were necessary for induction of polyclonal antibody-secreting cells by all three stimuli.
当用2型肺炎链球菌R36a的粗糙型在体外刺激外周血淋巴细胞（PBL）时，产生的空斑形成细胞（PFC）不会产生针对磷酰胆碱的抗体，磷酰胆碱是细胞壁C多糖的主要抗原决定簇。相反，R36a刺激PBL和脾淋巴细胞中的多克隆PFC。我们将R36a刺激的多克隆反应与两种特征明确的多克隆激活剂（PA）——金黄色葡萄球菌考恩I和商陆丝裂原（PWM）诱导的反应进行了比较。我们发现，R36a对PBL而言是一种低效的丝裂原，而另外两种PA是强效丝裂原；对所有三种PA产生反应的主要同种型是IgM；贴壁细胞强烈抑制对R36a和金黄色葡萄球菌的多克隆PFC反应，但不抑制对PWM的反应；并且T细胞对于所有三种刺激诱导多克隆抗体分泌细胞是必需的。
