Azzone G F, Pozzan T, Massari S, Bragadin M
Biochim Biophys Acta. 1978 Feb 9;501(2):296-306. doi: 10.1016/0005-2728(78)90035-x.
The correlation between deltamuH, the proton electrochemical potential difference, and the rate of controlled respiration is analyzed. deltamuH (the proton concentration gradient) is measured on the distribution of [3H]acetate, and deltapsi (the membrane potential) on the distribution of 86Rb+, 45Ca2+ and [3H]triphenylmethylphosphonium used either alone or simultaneously. The effects of the addition of ADP + hexokinase (state-3 ADP) and of carbonylcyanide trifluoromethoxyphenylhydrazone (state-3 uncoupler) on respiration and deltamuH are not equivalent: the uncoupler depresses deltamuH more than ADP at equivalent respiratory rates. The effects of the additions of nigericin-valinomycin and of ionophore A23187 (state-3 cation transport) and of carbonylcyanide trifluoromethoxy-phenylhydrazone (state 3-uncoupler) on respiration and deltamuH are also not equivalent: the uncoupler depresses deltamuH more than A23187 and nigericin + valinomycin at equivalent respiratory rate. A23187 is very efficient in stimulating respiration with negligible deltamuH changes.
分析了质子电化学势差ΔμH与受控呼吸速率之间的相关性。通过[3H]乙酸盐的分布来测量ΔμH（质子浓度梯度），并通过单独或同时使用86Rb+、45Ca2+和[3H]三苯基甲基鏻的分布来测量Δψ（膜电位）。添加ADP+己糖激酶（状态3 ADP）和羰基氰化物三氟甲氧基苯腙（状态3解偶联剂）对呼吸作用和ΔμH的影响并不相同：在同等呼吸速率下，解偶联剂对ΔμH的抑制作用比ADP更强。添加尼日利亚菌素-缬氨霉素、离子载体A23187（状态3阳离子转运）和羰基氰化物三氟甲氧基苯腙（状态3解偶联剂）对呼吸作用和ΔμH的影响也不相同：在同等呼吸速率下解偶联剂对ΔμH的抑制作用比A23187以及尼日利亚菌素+缬氨霉素更强。A23187在刺激呼吸作用方面非常有效，而对ΔμH的改变可忽略不计。
