Koide Y, Yoshida T O
Immunol Lett. 1984;7(4):209-13. doi: 10.1016/0165-2478(84)90045-2.
Two of three distinct human Ia molecules detected by murine monoclonal antibodies (MoAb) have been suggested to be involved in antigen presentation for T cell responses to purified protein derivatives (PPD) and herpes simplex virus (HSV). This observation was first suggested from studies on the inhibition of proliferative responses of whole T cell populations with MoAb against human Ia molecules. To determine whether a single T cell recognizes the antigen in the context of both Ia molecules or in the context of each one of two Ia molecules, we isolated and propagated PPD-reactive T cell clones from an HLA-DR heterozygous individual. They showed four different restriction patterns: type I and type II clones each appeared to be restricted to one of two HLA-DR antigens, type III clone gave anomalous patterns of response and seemed to be restricted to non-DR antigens, and type IV clone recognized antigen when both DR antigens were presented on the same antigen-presenting cells (APC) surface. Blocking study with monoclonal anti-Ia antibodies suggested that type I, II and IV clones are restricted to DR molecules and type III clones are restricted to 1B4 molecules distinct from DR or MB1 molecules. These data imply that human T cell clones recognizing PPD in the context of each one of two Ia molecules are clonally distributed.
鼠单克隆抗体（MoAb）检测到的三种不同人类Ia分子中的两种，被认为参与了针对纯化蛋白衍生物（PPD）和单纯疱疹病毒（HSV）的T细胞应答的抗原呈递。这一观察结果最初来自于用针对人类Ia分子的MoAb对整个T细胞群体增殖反应抑制的研究。为了确定单个T细胞是在两种Ia分子的背景下识别抗原，还是在两种Ia分子各自的背景下识别抗原，我们从一名HLA-DR杂合个体中分离并扩增了PPD反应性T细胞克隆。它们表现出四种不同的限制模式：I型和II型克隆似乎各自被限制于两种HLA-DR抗原中的一种，III型克隆给出异常的反应模式，似乎被限制于非DR抗原，IV型克隆在同一抗原呈递细胞（APC）表面同时呈现两种DR抗原时识别抗原。用单克隆抗Ia抗体进行的阻断研究表明，I型、II型和IV型克隆被限制于DR分子，III型克隆被限制于与DR或MB1分子不同的1B4分子。这些数据表明，在两种Ia分子各自的背景下识别PPD的人类T细胞克隆是克隆性分布的。
