Koide Y, Yoshida T O
Hum Immunol. 1984 Aug;10(4):203-12. doi: 10.1016/0198-8859(84)90086-7.
Participation of two of three distinct human Ia molecules, HLA-DR and the Ia molecule detected by monoclonal antibody (MoAb) 1B4 (1B4 molecules), in antigen presentation for T cell responses to purified protein derivative (PPD) and herpes simplex virus (HSV) was first suggested from studies on the inhibition of proliferative responses of whole T cell populations with MoAb against human Ia molecules. To determine whether a single T cell recognizes the antigen in the context of both Ia molecules or in the context of each one of two Ia molecules, we isolated and propagated PPD-reactive T cell clones from an HLA-DR heterozygous individual. The clones showed four different restriction patterns: type I and type II clones appeared to be restricted to one of two HLA-DR antigens, type III clones gave anomalous patterns of response and seemed to be restricted to non-DR antigens, and type IV clone recognized antigen when both DR antigens were presented on the same APC surface. Blocking study with MoAb to Ia molecules suggested that type I and type II clones are restricted to DR molecules and type III clones are restricted to 1B4 molecules distinct from DR or MB1 molecules. Furthermore, it is most likely that type IV clone was restricted to the interaction molecule associated with DR antigens. These data imply that human T cell clones recognizing PPD in the context of each one of two Ia molecules are clonally distinct.
通过针对人类Ia分子的单克隆抗体（MoAb）抑制全T细胞群体的增殖反应的研究，首次表明三种不同的人类Ia分子中的两种，即HLA-DR和由单克隆抗体1B4（1B4分子）检测到的Ia分子，参与了对纯化蛋白衍生物（PPD）和单纯疱疹病毒（HSV）的T细胞反应的抗原呈递。为了确定单个T细胞是在两种Ia分子的背景下识别抗原，还是在两种Ia分子各自的背景下识别抗原，我们从一名HLA-DR杂合个体中分离并培养了PPD反应性T细胞克隆。这些克隆表现出四种不同的限制模式：I型和II型克隆似乎仅限于两种HLA-DR抗原中的一种，III型克隆给出异常的反应模式，似乎仅限于非DR抗原，IV型克隆在同一APC表面呈现两种DR抗原时识别抗原。用针对Ia分子的MoAb进行的阻断研究表明，I型和II型克隆仅限于DR分子，III型克隆仅限于与DR或MB1分子不同的1B4分子。此外，IV型克隆很可能仅限于与DR抗原相关的相互作用分子。这些数据表明，在两种Ia分子各自的背景下识别PPD的人类T细胞克隆在克隆上是不同的。
