Higgins P J, Piwnicka M, Darzynkiewicz Z, Melamed M R
Am J Pathol. 1984 Apr;115(1):31-5.
Combined immunohistologic and flow cytometric methods were used to monitor the regenerative response of the mouse liver following intraperitoneal administration of the hepatonecrotic agent carbon tetrachloride (CCl4). A slight increase in the percentage of liver nuclei engaged in DNA synthesis (S-phase population) was evident 1 day after CCl4. The percentage of S-phase nuclei increased thereafter, lasting until day 6 after CCl4 with a peak at day 2 after CCl4. The method of sampling used (every 24 hours) places the period of maximal DNA synthetic activity between 48 hours and 72 hours after inoculation of the hepatonecrotic agent; at 48 hours the number of S-phase nuclei was approximately five times higher than the nonregenerating liver. Modified multiparametric flow cytofluorimetric analysis of hepatic nuclei indicated that early repopulation of the liver in response to CCl4-induced necrosis involved the selective proliferation of a specific subpopulation of liver cells which contained diploid nuclei of high RNA content. These nuclei were also observed in control animals, suggesting the existence of a subcompartment of 2C DNA content G1 hepatocytes potentially programmed for replacement proliferation.
采用免疫组织化学和流式细胞术相结合的方法，监测腹腔注射肝坏死剂四氯化碳（CCl4）后小鼠肝脏的再生反应。CCl4注射1天后，参与DNA合成的肝细胞核百分比（S期群体）略有增加。此后，S期细胞核的百分比持续上升，直到CCl4注射后第6天，在CCl4注射后第2天达到峰值。所采用的取样方法（每24小时一次）表明，在接种肝坏死剂后48小时至72小时之间出现最大DNA合成活性；在48小时时，S期细胞核的数量比未再生的肝脏高出约五倍。对肝细胞核进行改良的多参数流式细胞荧光分析表明，肝脏对CCl4诱导的坏死的早期再填充涉及特定肝细胞亚群的选择性增殖，这些肝细胞含有高RNA含量的二倍体细胞核。在对照动物中也观察到了这些细胞核，这表明存在一个2C DNA含量的G1肝细胞亚群，可能被编程用于替代增殖。
