Mangels J I, Cox M E, Lindberg L H
Diagn Microbiol Infect Dis. 1984 Apr;2(2):129-37. doi: 10.1016/0732-8893(84)90008-7.
Methanol fixation of Gram-stained smears was compared to heat fixation. Smears were prepared in duplicate from direct clinical specimens, blood culture bottles, and bacterial colonies. Results from this study show that methanol fixation is superior in every instance to heat fixation. The morphology of bacteria and tissue cells was not distorted, little or no background debris was observed, and a better Gram reaction was noted.
将革兰氏染色涂片的甲醇固定法与热固定法进行了比较。从直接临床标本、血培养瓶和细菌菌落中制备了一式两份的涂片。这项研究的结果表明，在每种情况下，甲醇固定法都优于热固定法。细菌和组织细胞的形态没有扭曲，观察到很少或没有背景碎片，并且革兰氏反应更好。
