Magee C M, Rodeheaver G, Edgerton M T, Edlich R F
Am J Surg. 1975 Sep;130(3):341-6. doi: 10.1016/0002-9610(75)90398-0.
The purpose of this study was to identify pitfalls in the Gram staining technic that limit its diagnostic value. In our clinical experience, gram-positive organisms were often decolorized too easily. Factors have been identified that alter the susceptibility of gram-positive organisms to decolorization in the Gram staining technic. The age of the bacterial culture, the preparation of the smear, the fixation technic, and the mordant have an important influence on the ease with which gram-positive organisms are decolorized. On the basis of these studies, a more reliable and reproducible Gram staining technic has been developed for the diagnosis of surgical infections.
本研究的目的是确定革兰氏染色技术中限制其诊断价值的缺陷。根据我们的临床经验，革兰氏阳性菌常常很容易被脱色。已确定在革兰氏染色技术中会改变革兰氏阳性菌对脱色敏感性的因素。细菌培养物的菌龄、涂片制备、固定技术和媒染剂对革兰氏阳性菌被脱色的难易程度有重要影响。基于这些研究，已开发出一种更可靠且可重复的革兰氏染色技术用于外科感染的诊断。
