Forre O, Natvig J B, Kunkel H G
J Exp Med. 1976 Oct 1;144(4):897-905. doi: 10.1084/jem.144.4.897.
Serological test systems were established for determining the heavy-chain variable region (Vh) subgroups of immunoglobulin heavy chains. Myeloma proteins with known Vh subgroups based on amino acid sequence were utilized as the primary basis of reference for analysis by hemagglutination and hemagglutination inhibition. Good agreement between the chemical and serological typing was obtained and nonoverlapping systems established for the three major Vh subgroups. In a survey of 167 myeloma proteins, all except two were exclusively positive in one of the three systems. The two exceptions may represent a fourth subgroup. There was an overall incidence ratio of 1:2:3 for VhI:VhII:VhIII subgroups. Some differences in the overall ratios were encountered within the immunoglobulin classes. Certain advantages of the serological typing antisera were discussed with special emphasis on their use for studies of Vh antigens on the membranes of lymphocytes.
建立了血清学检测系统来确定免疫球蛋白重链的重链可变区(Vh)亚群。基于氨基酸序列具有已知Vh亚群的骨髓瘤蛋白被用作血凝和血凝抑制分析的主要参考依据。化学分型和血清学分型之间取得了良好的一致性,并为三个主要的Vh亚群建立了不重叠的系统。在对167种骨髓瘤蛋白的调查中,除了两种蛋白外,其他所有蛋白在这三个系统中的一个中均呈阳性。这两个例外可能代表第四个亚群。VhI:VhII:VhIII亚群的总体发生率为1:2:3。在免疫球蛋白类别中,总体比例存在一些差异。讨论了血清学分型抗血清的某些优点,特别强调了它们在研究淋巴细胞膜上Vh抗原方面的用途。