通过酶联免疫吸附测定（ELISA）对解脲脲原体菌株进行血清学鉴定。
Serological characterisation of Ureaplasma urealyticum strains by enzyme-linked immunosorbent assay (ELISA).
作者信息
Turunen H, Leinikki P, Jansson E
出版信息
J Clin Pathol. 1982 Apr;35(4):439-43. doi: 10.1136/jcp.35.4.439.
Abstract
A modification of enzyme-linked immunosorbent assay (ELISA) was developed for the serological characterisation and identification of strains of Ureaplasma urealyticum. The eight recognised human serotypes of U urealyticum and antisera produced against them were used as reference for the evaluation and standardisation of the method. The serological profile illustrating reactions of antigen with homologous and heterologous antisera was specific and reproducible for each serotype. The homologous reaction was always very prominent but some cross-reactivity was seen, most clearly between serotypes 2 and 5. The method was found to be suitable for serological typing of clinical isolates of U urealyticum because of rapid and simple technical procedure, good reproducibility of the results and economical consumption of antisera and other reagents.
摘要
开发了一种酶联免疫吸附测定（ELISA）的改良方法，用于解脲脲原体菌株的血清学鉴定和分型。将公认的8种解脲脲原体人类血清型及其相应抗血清用作该方法评估和标准化的参考。每种血清型的抗原与同源和异源抗血清反应的血清学图谱具有特异性且可重复。同源反应总是非常显著，但也观察到一些交叉反应，最明显的是血清型2和5之间。由于该方法技术操作快速简便、结果重现性好且抗血清和其他试剂消耗经济，因此被认为适用于解脲脲原体临床分离株的血清学分型。
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