DeWitt C M, Field A K
J Interferon Res. 1984;4(1):1-8. doi: 10.1089/jir.1984.4.1.
Grivet monkeys immunosuppressed with either cyclophosphamide or methylprednisolone retain their capacity to produce interferon in response to poly I:C/poly-L-lysine (poly ICL). To stimulate a human immunosuppressed condition, monkeys were maintained in a lymphocyte suppressed state (500-2200 lymphocytes/mm3, mean = 1550) for five weeks by weekly intravenous injections of cyclophosphamide (50 mg/kg i.v.). Monkeys similarly treated with phosphate buffered saline retained lymphocytes within the normal range (3900-10,000 lymphocytes/mm3, mean = 8320). Both groups produced comparable interferon (IFN) titers in response to weekly induction (iv) with poly ICL. Monkeys injected once with methylprednisolone (15 mg/kg iv) and then induced with poly ICL 4 h later (maximum lymphocyte suppression) retained their full capacity to produce IFN. However, repeated injections of poly ICL in normal grivet monkeys resulted in hyporesponsive IFN production following daily or alternate day injections, but not following injections every third day. Total IFN produced was similar regardless of the frequency of inducer injections. All IFNs produced were similar to human leukocyte interferon (HuIFN-alpha); e.g., higher antiviral titers on bovine cells than human fibroblasts and neutralization with antisera against IFN-alpha.
用环磷酰胺或甲基强的松龙免疫抑制的狮尾狒狒，在对聚肌胞苷酸/聚-L-赖氨酸（聚ICL）产生反应时仍保留产生干扰素的能力。为了模拟人类免疫抑制状态，通过每周静脉注射环磷酰胺（50mg/kg静脉注射），使猴子维持淋巴细胞抑制状态（500 - 2200个淋巴细胞/mm³，平均 = 1550）达五周。用磷酸盐缓冲盐水进行类似处理的猴子，其淋巴细胞数量保持在正常范围内（3900 - 10,000个淋巴细胞/mm³，平均 = 8320）。两组在每周用聚ICL诱导（静脉注射）后产生的干扰素（IFN）滴度相当。单次注射甲基强的松龙（15mg/kg静脉注射），然后在4小时后用聚ICL诱导（最大程度抑制淋巴细胞）的猴子，仍保留产生IFN的全部能力。然而，在正常狮尾狒狒中重复注射聚ICL，每日或隔日注射后会导致IFN产生反应低下，但每三天注射一次则不会。无论诱导剂注射频率如何，产生的总IFN量相似。产生的所有IFN都与人白细胞干扰素（HuIFN-α）相似；例如，在牛细胞上的抗病毒滴度高于人成纤维细胞，并且能用抗IFN-α抗血清进行中和。
