Jha R S, Wickenden C, Anderson M C, Coleman D V
Br J Obstet Gynaecol. 1984 May;91(5):483-8. doi: 10.1111/j.1471-0528.1984.tb04788.x.
Five monoclonal antibodies (Ca1, HMFG 1 and 2, 8.30.3 and 77.1) were used to study the distribution of antibody binding sites in cervical tissue with a view to identifying a marker which would distinguish between benign and malignant cervical epithelium. Both benign tissue (mature and immature metaplastic squamous epithelium, congenital transformation zone and glandular epithelium) and neoplastic tissue (cervical intraepithelial neoplasia, 1, 2 and 3 and invasive squamous cell carcinoma) were stained by these antibodies. Although immature metaplastic epithelium stained strongly with all the antibodies, the intensity and distribution of staining in general did not distinguish between benign and neoplastic conditions. All five antibodies, raised against three different antigens, stained cervical tissue in a similar way and thus were unsuitable for use as specific tumour markers in equivocal cases. Further studies on other tumour markers are indicated in view of the potential value of this approach.
使用五种单克隆抗体（Ca1、HMFG 1和2、8.30.3以及77.1）研究宫颈组织中抗体结合位点的分布，旨在识别一种能够区分宫颈良性和恶性上皮的标志物。良性组织（成熟和未成熟化生鳞状上皮、先天性转化区和腺上皮）和肿瘤组织（宫颈上皮内瘤变1、2和3级以及浸润性鳞状细胞癌）均用这些抗体进行染色。尽管未成熟化生上皮与所有抗体均有强烈染色，但总体染色强度和分布并不能区分良性和肿瘤状态。针对三种不同抗原产生的所有五种抗体，对宫颈组织的染色方式相似，因此在疑难病例中不适用于作为特异性肿瘤标志物。鉴于这种方法的潜在价值，有必要对其他肿瘤标志物进行进一步研究。
