人乳头多瘤空泡病毒RFV的特性：与猴空泡病毒40和BK病毒的比较

Characterization of human papovavirus RFV: comparison with SV40 and BKV.

作者信息

Miao R, Dougherty R M

出版信息

J Gen Virol. 1977 Apr;35(1):67-75. doi: 10.1099/0022-1317-35-1-67.

Abstract

Human papovavirus, RFV, isolated from urine of a renal transplant patient was compared with two strains of SV40 and with the prototype human papovavirus, BKV. Neutralization tests showed that RFV and BKV are indistinguishable, while large-plaque (LP) and small-plaque(SP) isolates of SV40 gave a low but significant level of cross-reaction with rabbit or human antisera against RFV. DNA reassociation saturation tests using 125I-labelled RFV DNA show that BKV has 88% homology, and SP-SV40 has 29% homology to RFV. We conclude that RFV and BKV are nearly, if not totally, identical and are not SV40 variants.

摘要

从一名肾移植患者尿液中分离出的人多瘤病毒RFV，与两株SV40以及人多瘤病毒原型BKV进行了比较。中和试验表明，RFV和BKV无法区分，而SV40的大斑（LP）和小斑（SP）分离株与兔或人抗RFV血清有低但显著水平的交叉反应。使用125I标记的RFV DNA进行的DNA重缔合饱和试验表明，BKV与RFV有88%的同源性，SP-SV40与RFV有29%的同源性。我们得出结论，RFV和BKV即使不完全相同也几乎相同，且不是SV40变体。