Toyama S, Toyama S
Cell. 1984 Jun;37(2):609-14. doi: 10.1016/0092-8674(84)90391-x.
Lines of KB cells resistant to cytochalasin B have been isolated and characterized. The mutant Cyt 1 exhibits increased cross-resistance to cytochalasin E. Cyt 1 cells bind less cytochalasin B than parental KB cells. Two-dimensional gel analysis shows that Cyt 1 cells carry an alteration in beta-actin. This is further confirmed by one-dimensional peptide analysis. The altered beta-actin (beta'-actin) is synthesized when poly(A)+ RNA from Cyt 1 cells is translated in a reticulocyte-cell-free translation system. These results suggest that the primary site of action of cytochalasin B on cellular motility processes is beta-actin.
已分离并鉴定出对细胞松弛素B具有抗性的KB细胞系。突变体Cyt 1对细胞松弛素E表现出增加的交叉抗性。Cyt 1细胞比亲本KB细胞结合的细胞松弛素B更少。二维凝胶分析表明Cyt 1细胞的β-肌动蛋白发生了改变。一维肽分析进一步证实了这一点。当在网织红细胞无细胞翻译系统中翻译来自Cyt 1细胞的聚腺苷酸加尾RNA时，会合成改变的β-肌动蛋白（β'-肌动蛋白）。这些结果表明细胞松弛素B对细胞运动过程的主要作用位点是β-肌动蛋白。
