Detection limits for protein after electrophoresis on cellulose acetate.
作者信息
Goodland F C
出版信息
Ann Clin Biochem. 1982 Mar;19(Pt 2):117-9. doi: 10.1177/000456328201900211.
PMID:7073213
Abstract
The detection limits for protein, when stained with nigrosine after electrophoresis on cellulose acetate, was studied and shown to be: albumin, 3-0 ng; transferrin, 0.8 ng; and Bence-Jones protein, 0.8 ng. This was less than one-hundredth of the previously reported levels. Immunofixation, although useful for identification, did not lower the detection limit for Bence-Jones protein.
摘要
相似文献
1
Detection limits for protein after electrophoresis on cellulose acetate.
Ann Clin Biochem. 1982 Mar;19(Pt 2):117-9. doi: 10.1177/000456328201900211.
2
3
4
[Method of separating proteins and isoenzymes on cellulose acetate films].
Lab Delo. 1981(3):146-8.
5
"Immunosubtraction" electrophoresis: a simple method for identifying specific proteins producing the cellulose acetate electrophoretogram.“免疫扣除”电泳法：一种识别产生醋酸纤维素电泳图谱的特定蛋白质的简单方法。
Boll Ist Sieroter Milan. 1977 Jul 31;56(3):212-6.
6
[Modified albumin as a diagnostic and prognostic test].[改良白蛋白作为一种诊断和预后检测方法]
Vopr Onkol. 1988;34(9):1123-5.
7
Some applications of gel isoelectric focusing to the examination of human serum proteins.凝胶等电聚焦在人血清蛋白检测中的一些应用。
Proc R Soc Med. 1971 Jun;64(6):645-6. doi: 10.1177/003591577106400626.
8
[Detection of di-albuminemias using cellulose acetate electrophoresis].[使用醋酸纤维素电泳检测双白蛋白血症]
Z Med Lab Diagn. 1977 Feb 1;18(1):38-9.
9
引用本文的文献
1
Protein Identification and Quantification Using Porous Silicon Arrays, Optical Measurements, and Machine Learning.使用多孔硅阵列、光学测量和机器学习进行蛋白质鉴定和定量分析。
Biosensors (Basel). 2023 Sep 9;13(9):879. doi: 10.3390/bios13090879.
2
Identification of Markers for Root Traits Related to Drought Tolerance Using Traditional Rice Germplasm.利用传统水稻种质资源鉴定与耐旱性相关的根性状标记。
Mol Biotechnol. 2021 Dec;63(12):1280-1292. doi: 10.1007/s12033-021-00380-1. Epub 2021 Aug 16.
3
A comparison of cellulose acetate immunofixation with polyacrylamide gel electrophoresis for the detection of oligoclonal bands in unconcentrated cerebrospinal fluid.醋酸纤维素免疫固定法与聚丙烯酰胺凝胶电泳法在检测未浓缩脑脊液中寡克隆带方面的比较。
J Clin Pathol. 1983 Nov;36(11):1309-11. doi: 10.1136/jcp.36.11.1309.
Zotero 插件