通过降低细胞质pH值来扩增粪肠球菌质子转运ATP酶。
Amplification of the Streptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH.
作者信息
Kobayashi H, Suzuki T, Kinoshita N, Unemoto T
出版信息
J Bacteriol. 1984 Jun;158(3):1157-60. doi: 10.1128/jb.158.3.1157-1160.1984.
Abstract
When Streptococcus faecalis was grown in the presence of protonophores , an ATPase activity of the membrane was increased at a pH below 8.0 but not at a pH above 8.0. Characteristics of this increased ATPase were identical to those of a proton-translocating ATPase (H+-ATPase) located on the membrane of normal cells. The cytoplasmic pH was regulated at 7.6 to 7.8 but was not regulated in the presence of protonophores . The increase in the H+-ATPase was observed when the cytoplasmic pH was lowered to less than 7.6 by the addition of protonophores and was not related to the dissipation of the proton motive force. Thus, we suggest that the H+-ATPase of the membrane is amplified when the cytoplasmic pH is lowered below the pH at which it is regulated under normal conditions.
摘要
当粪肠球菌在质子载体存在的情况下生长时，膜的ATP酶活性在pH低于8.0时增加，而在pH高于8.0时不增加。这种增加的ATP酶的特性与正常细胞膜上的质子转运ATP酶（H⁺-ATP酶）相同。细胞质pH被调节在7.6至7.8，但在质子载体存在时不被调节。当通过添加质子载体使细胞质pH降低至低于7.6时，观察到H⁺-ATP酶增加，且这与质子动力势的耗散无关。因此，我们认为当细胞质pH降低到低于正常条件下其被调节的pH时，膜的H⁺-ATP酶会被放大。
相似文献
1
Amplification of the Streptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH.通过降低细胞质pH值来扩增粪肠球菌质子转运ATP酶。
J Bacteriol. 1984 Jun;158(3):1157-60. doi: 10.1128/jb.158.3.1157-1160.1984.
2
A proton-translocating ATPase regulates pH of the bacterial cytoplasm.一种质子转运ATP酶调节细菌细胞质的pH值。
J Biol Chem. 1985 Jan 10;260(1):72-6.
3
Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-translocating ATPase.链球菌的细胞质pH值通过质子转运ATP酶的数量和活性变化来调节。
J Biol Chem. 1986 Jan 15;261(2):627-30.
4
Regulation of the cytoplasmic pH by a proton-translocating ATPase in Streptococcus faecalis (faecium). A computer simulation.粪肠球菌（屎肠球菌）中质子转运ATP酶对细胞质pH的调节。计算机模拟。
Eur J Biochem. 1989 Mar 15;180(2):467-71. doi: 10.1111/j.1432-1033.1989.tb14669.x.
5
Mutants of Streptococcus faecalis sensitive to alkaline pH lack Na(+)-ATPase.对碱性pH敏感的粪肠球菌突变体缺乏钠-ATP酶。
J Bacteriol. 1990 Apr;172(4):1732-5. doi: 10.1128/jb.172.4.1732-1735.1990.
6
Novel streptococcal mutants defective in the regulation of H+-ATPase biosynthesis and in F0 complex.在H⁺-ATP酶生物合成调控和F0复合体方面存在缺陷的新型链球菌突变体。
J Biol Chem. 1988 Aug 25;263(24):11840-3.
7
Enzyme level of enterococcal F1Fo-ATPase is regulated by pH at the step of assembly.肠球菌F1Fo - ATP酶的酶水平在组装步骤受pH调节。
Eur J Biochem. 1999 Jan;259(1-2):262-8. doi: 10.1046/j.1432-1327.1999.00031.x.
8
Maintenance of a neutral cytoplasmic pH is not obligatory for growth of Escherichia coli and Streptococcus faecalis at an alkaline pH.
J Biochem. 1990 Jul;108(1):86-91. doi: 10.1093/oxfordjournals.jbchem.a123168.
9
[Regulatory mechanism of the cytoplasmic pH by H(+)-adenosinetriphosphatase].[H(+)-三磷酸腺苷酶对细胞质pH的调节机制]
Yakugaku Zasshi. 1990 Oct;110(10):703-10. doi: 10.1248/yakushi1947.110.10_703.
10
Altered expression of the H+ ATPase in Streptococcus faecalis membranes.粪肠球菌膜中H⁺ATP酶的表达改变。
Biochem Biophys Res Commun. 1984 Jul 18;122(1):151-7. doi: 10.1016/0006-291x(84)90452-2.
引用本文的文献
1
Integrating highly quantitative proteomics and genome-scale metabolic modeling to study pH adaptation in the human pathogen .整合高度定量蛋白质组学和基因组规模代谢模型以研究人类病原体中的pH适应性
NPJ Syst Biol Appl. 2016 Sep 8;2:16017. doi: 10.1038/npjsba.2016.17. eCollection 2016.
2
Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't.变形链球菌的酸适应性机制——我们知道得越多，未知的就越多。
Mol Oral Microbiol. 2017 Apr;32(2):107-117. doi: 10.1111/omi.12162. Epub 2016 Jun 21.
3
Adaptive responses of Bacillus cereus ATCC14579 cells upon exposure to acid conditions involve ATPase activity to maintain their internal pH.蜡样芽孢杆菌ATCC14579细胞在暴露于酸性条件下的适应性反应涉及ATP酶活性以维持其内部pH值。
Microbiologyopen. 2015 Apr;4(2):313-322. doi: 10.1002/mbo3.239. Epub 2015 Mar 5.
4
Na(+) as coupling ion in energy transduction in extremophilic Bacteria and Archaea.极端嗜盐古菌和细菌中 Na(+)作为能量传递的偶联离子。
World J Microbiol Biotechnol. 1995 Jan;11(1):58-70. doi: 10.1007/BF00339136.
5
Proton motive force in Rhodobacter sphaeroides under anaerobic conditions in the dark.厌氧黑暗条件下球形红杆菌中的质子动力。
Curr Microbiol. 2011 Feb;62(2):415-9. doi: 10.1007/s00284-010-9723-1. Epub 2010 Jul 24.
6
The increase in the number of accessible SH-groups in the enterococcal membrane vesicles by ATP and nicotinamide adenine dinucleotides.ATP和烟酰胺腺嘌呤二核苷酸使肠球菌膜囊泡中可及巯基数量增加。
Curr Microbiol. 2006 Apr;52(4):300-4. doi: 10.1007/s00284-005-0293-6. Epub 2006 Mar 18.
7
Acid-inducible transcription of the operon encoding the citrate lyase complex of Lactococcus lactis Biovar diacetylactis CRL264.乳酸乳球菌双乙酰亚种CRL264中编码柠檬酸裂解酶复合体的操纵子的酸诱导转录。
J Bacteriol. 2004 Sep;186(17):5649-60. doi: 10.1128/JB.186.17.5649-5660.2004.
8
Bifidobacterium lactis DSM 10140: identification of the atp (atpBEFHAGDC) operon and analysis of its genetic structure, characteristics, and phylogeny.乳酸双歧杆菌DSM 10140：atp（atpBEFHAGDC）操纵子的鉴定及其遗传结构、特征和系统发育分析。
Appl Environ Microbiol. 2004 May;70(5):3110-21. doi: 10.1128/AEM.70.5.3110-3121.2004.
9
Surviving the acid test: responses of gram-positive bacteria to low pH.经受严峻考验：革兰氏阳性菌对低pH值的反应
Microbiol Mol Biol Rev. 2003 Sep;67(3):429-53, table of contents. doi: 10.1128/MMBR.67.3.429-453.2003.
10
Membrane-bound ATPase contributes to hop resistance of Lactobacillus brevis.膜结合ATP酶有助于短乳杆菌的啤酒花抗性。
Appl Environ Microbiol. 2002 Nov;68(11):5374-8. doi: 10.1128/AEM.68.11.5374-5378.2002.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Regulation of the cytoplasmic pH in Streptococcus faecalis.粪肠球菌胞质pH的调节
J Biol Chem. 1982 Nov 25;257(22):13246-52.
3
Energy coupling to potassium transport in Streptococcus faecalis. Interplay of ATP and the protonmotive force.粪肠球菌中能量与钾转运的偶联。ATP与质子动力的相互作用。
J Biol Chem. 1980 Jan 25;255(2):433-40.
4
ATP-driven sodium pump in Streptococcus faecalis.粪肠球菌中的ATP驱动钠泵。
Proc Natl Acad Sci U S A. 1982 May;79(9):2798-802. doi: 10.1073/pnas.79.9.2798.
5
Interconversion of components of the bacterial proton motive force by electrogenic potassium transport.通过生电钾转运实现细菌质子动力势各组分的相互转化。
J Bacteriol. 1981 Sep;147(3):820-6. doi: 10.1128/jb.147.3.820-826.1981.
6
Streptococcus faecalis mutants defective in regulation of cytoplasmic pH.粪肠球菌在细胞质pH调节方面存在缺陷的突变体。
J Bacteriol. 1980 Sep;143(3):1187-93. doi: 10.1128/jb.143.3.1187-1193.1980.
7
Purification of a putative K+-ATPase from Streptococcus faecalis.从粪肠球菌中纯化一种假定的钾离子-ATP酶
J Biol Chem. 1983 Jun 25;258(12):7611-7.
8
Constitutive inorganic pyrophosphatase of Escherichia coli. 1. Purification and catalytic properties.大肠杆菌的组成型无机焦磷酸酶。1. 纯化及催化特性。
J Biol Chem. 1966 May 10;241(9):1938-47.
9
A specific alkaline p-nitrophenylphosphatase activity from baker's yeast.来自面包酵母的一种特定碱性对硝基苯磷酸酶活性。
Biochim Biophys Acta. 1972 May 12;268(2):422-30. doi: 10.1016/0005-2744(72)90338-5.
10
Membrane-bound adenosine triphosphatase of Escherichia coli. I. Partial purification and properties.大肠杆菌的膜结合三磷酸腺苷酶。I. 部分纯化及性质
J Biochem. 1972 Mar;71(3):387-99.
Zotero 插件