Wathen M W, Wathen L M
J Virol. 1984 Jul;51(1):57-62. doi: 10.1128/JVI.51.1.57-62.1984.
A pseudorabies virus variant ( mar197 -1) containing a mutation in a viral glycoprotein with a molecular weight of 50,000 ( gp50 ) was isolated by selecting for resistance to a neurtralizing monoclonal antibody ( MCA50 -1) directed against gp50 . This mutant was completely resistant to neutralization with MCA50 -1 in the presence or absence of complement, and was therefore defined as a mar (monoclonal-antibody-resistant) mutant. The mutation did not affect neutralization with polyvalent immune serum. The mar197 -1 mutant synthesized and processed gp50 normally, but the mutation prevented the binding and immunoprecipitation of gp50 by MCA50 -1. Thus, the mutation was within the structural portion of the gp50 gene affecting the epitope of the monoclonal antibody. The mutation was mapped by marker rescue with cloned pseudorabies restriction enzyme fragments to the short region of the pseudorabies genome between 0.813 and 0.832 map units. This is equivalent to a 2.1-kilobase-pair region.
通过筛选对针对分子量为50,000的病毒糖蛋白（gp50）的中和单克隆抗体（MCA50 -1）具有抗性，分离出了一种伪狂犬病病毒变体（mar197 -1），该变体在gp50中存在突变。在有或没有补体的情况下，此突变体对MCA50 -1的中和作用完全具有抗性，因此被定义为mar（单克隆抗体抗性）突变体。该突变不影响用多价免疫血清进行的中和作用。mar197 -1突变体正常合成和加工gp50，但该突变阻止了MCA50 -1对gp50的结合和免疫沉淀。因此，该突变位于gp50基因的结构部分内，影响单克隆抗体的表位。通过用克隆的伪狂犬病限制酶片段进行标记拯救，将该突变定位到伪狂犬病基因组中位于0.813和0.832图谱单位之间的短区域。这相当于一个2.1千碱基对的区域。
