Weis J H, Enquist L W, Salstrom J S, Watson R J
Nature. 1983 Mar 3;302(5903):72-4. doi: 10.1038/302072a0.
Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) cause both persistent and latent infections, including recurrent cutaneous disease, lethal neonatal disease, central nervous system disease and other clinical syndromes. Modified live vaccines or conventionally prepared subunit vaccines have generally been unsuccessful in the treatment of HSV-1 and HSV-2 infections from the standpoints of safety and efficacy. It has been established that HSV-1 and HSV-2 infectivity may be neutralized in vitro with antisera directed specifically against each of the four major glycoproteins of the virus (gA/gB, gC, gD and gE) and antisera against glycoprotein gD, of either HSV-1 or HSV-2, are capable of neutralizing both HSV-1 and HSV-2 infectivity in vitro and in vivo. We have previously reported on the identification, DNA sequence and expression at low level in Escherichia coli of the gD gene of HSV-1 strain Patton. Here we describe construction of a hybrid gene encoding a chimaeric protein containing HSV-1 gD, bacteriophage lambda Cro and E. coli beta-galactosidase (gD-beta-gal) protein, which is expressed at high level in E. coli. Moreover, the chimaeric protein elicits antibodies in rabbits that not only immunoprecipitate gD from cells infected with HSV-1 and HSV-2 but also neutralize HSV-1 and HSV-2 infectivity in vitro.
1型单纯疱疹病毒（HSV - 1）和2型单纯疱疹病毒（HSV - 2）可引起持续性和潜伏性感染，包括复发性皮肤疾病、致死性新生儿疾病、中枢神经系统疾病及其他临床综合征。从安全性和有效性的角度来看，减毒活疫苗或传统制备的亚单位疫苗在治疗HSV - 1和HSV - 2感染方面通常并不成功。已经证实，HSV - 1和HSV - 2的感染性在体外可用特异性针对该病毒四种主要糖蛋白（gA/gB、gC、gD和gE）中每一种的抗血清中和，并且针对HSV - 1或HSV - 2糖蛋白gD的抗血清能够在体外和体内中和HSV - 1和HSV - 2的感染性。我们之前报道了HSV - 1帕顿株gD基因的鉴定、DNA序列及在大肠杆菌中的低水平表达。在此我们描述了一种杂交基因的构建，该基因编码一种包含HSV - 1 gD、噬菌体λ Cro和大肠杆菌β - 半乳糖苷酶（gD - β - gal）蛋白的嵌合蛋白，其在大肠杆菌中高水平表达。此外，该嵌合蛋白在兔体内引发的抗体不仅能从感染HSV - 1和HSV - 2的细胞中免疫沉淀gD，还能在体外中和HSV - 1和HSV - 2的感染性。
