Koch N, Sandrin M S, Hämmerling G J, Tada N, Kimura S, Hämmerling U
Mol Immunol. 1984 Apr;21(4):293-9. doi: 10.1016/0161-5890(84)90100-7.
Binding data on inbred mouse strains and immunochemical isolation of Ia antigens with subsequent separation on non-reduced/reduced two-dimensional gels provide evidence for the cross-reactivity of monoclonal antibodies with I-A and I-E products. Thus two monoclonal antibodies were found to react with A alpha A beta as well as E alpha E beta dimers. One of these mAbs, K22 -42, reacts with the precursor form of E beta chain of B10.GD mice which is associated with the invariant chain (Ii). This indicates that the respective determinant on E beta is formed prior to association of E beta with E alpha.
近交系小鼠品系的结合数据以及Ia抗原的免疫化学分离，随后在非还原/还原二维凝胶上进行分离，为单克隆抗体与I-A和I-E产物的交叉反应性提供了证据。因此，发现两种单克隆抗体与AαAβ以及EαEβ二聚体发生反应。其中一种单克隆抗体K22 -42与B10.GD小鼠Eβ链的前体形式发生反应，该前体形式与恒定链（Ii）相关。这表明Eβ上的相应决定簇在Eβ与Eα缔合之前就已形成。
