具有独特结构的人类U4 RNA假基因。

A pseudogene for human U4 RNA with a remarkable structure.

作者信息

Hammarström K, Westin G, Pettersson U

出版信息

EMBO J. 1982;1(6):737-9. doi: 10.1002/j.1460-2075.1982.tb01239.x.

DOI:10.1002/j.1460-2075.1982.tb01239.x

PMID:6203736

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553277/

Abstract

The human DNA library of Lawn et al. (1978) was screened for sequences complementary to the small nuclear (sn) RNA U4. Several positive clones were identified by screening 100 000 recombinants, indicating that U4 sequences like other snRNA sequences are dispersed in the human genome. One recombinant was characterized in detail by subcloning a Bg/II fragment 1.9 kilobases (kb) long in the pBR322 plasmid. The subcloned fragment was partially sequenced and the results revealed a pseudogene for U4 RNA. The pseudogene was found to have a remarkable structure; it contains a sequence that, except in two positions, matches the first 68 nucleotides of the human U4 RNA sequence and the pseudogene is, moreover, flanked by perfect direct repeats 20 bp long. The results support the model of van Arsdell et al. (1981) suggesting that certain snRNA pseudogenes arise by reverse transcription of the RNA followed by integration of the cDNA copy at a new chromosomal locus.

摘要

用人的DNA文库（Lawn等人，1978年）筛选与小核（sn）RNA U4互补的序列。通过筛选100,000个重组体鉴定出几个阳性克隆，这表明U4序列与其他snRNA序列一样分散在人类基因组中。通过在pBR322质粒中对一个1.9千碱基（kb）长的Bg/II片段进行亚克隆，对其中一个重组体进行了详细表征。对亚克隆片段进行了部分测序，结果揭示了一个U4 RNA的假基因。发现该假基因具有显著的结构；它包含一个序列，除了两个位置外，与人类U4 RNA序列的前68个核苷酸匹配，而且该假基因两侧是20 bp长的完美正向重复序列。这些结果支持了van Arsdell等人（1981年）的模型，该模型表明某些snRNA假基因是由RNA的逆转录产生，随后cDNA拷贝整合到新的染色体位点。

相似文献

A pseudogene for human U4 RNA with a remarkable structure.具有独特结构的人类U4 RNA假基因。

EMBO J. 1982;1(6):737-9. doi: 10.1002/j.1460-2075.1982.tb01239.x.

U4 small nuclear RNA pseudogenes from rat genome have common truncated 3'-ends.来自大鼠基因组的U4小核RNA假基因具有共同的截短3'末端。

Biochem Biophys Res Commun. 1985 Jul 31;130(2):828-34. doi: 10.1016/0006-291x(85)90491-7.

Nonrandom integration of human U4 RNA pseudogenes.人类U4 RNA假基因的非随机整合

Mol Cell Biol. 1985 May;5(5):943-8. doi: 10.1128/mcb.5.5.943-948.1985.

A candidate gene for human U1 RNA.人类U1 RNA的一个候选基因。

EMBO J. 1982;1(1):133-7. doi: 10.1002/j.1460-2075.1982.tb01136.x.

Pseudogenes for human U2 small nuclear RNA do not have a fixed site of 3' truncation.人类U2小核RNA的假基因没有固定的3'端截短位点。

Nucleic Acids Res. 1984 Feb 10;12(3):1463-71. doi: 10.1093/nar/12.3.1463.

Nucleotide sequence and organization of full length human U4 RNA pseudogenes.全长人类U4 RNA假基因的核苷酸序列与结构

Gene. 1989 Aug 15;80(2):385-9. doi: 10.1016/0378-1119(89)90304-1.

Direct repeats flank three small nuclear RNA pseudogenes in the human genome.在人类基因组中，直接重复序列位于三个小核RNA假基因的两侧。

Cell. 1981 Oct;26(1 Pt 1):11-7. doi: 10.1016/0092-8674(81)90028-3.

Abundant pseudogenes for small nuclear RNAs are dispersed in the human genome.小核RNA的大量假基因分散在人类基因组中。

Proc Natl Acad Sci U S A. 1981 Feb;78(2):810-4. doi: 10.1073/pnas.78.2.810.

Novel structure of a human U6 snRNA pseudogene.人类U6小核仁RNA假基因的新结构

Gene. 1985;36(1-2):195-9. doi: 10.1016/0378-1119(85)90086-1.

Organization of sequences related to U6 RNA in the human genome.人类基因组中与U6 RNA相关的序列组织

Nucleic Acids Res. 1981 Jul 24;9(14):3379-88. doi: 10.1093/nar/9.14.3379.

引用本文的文献

A plant pseudogene for U1 RNA.一种植物U1 RNA假基因。

Plant Mol Biol. 1989 Jun;12(6):709-11. doi: 10.1007/BF00044161.

BC1 RNA, the transcript from a master gene for ID element amplification, is able to prime its own reverse transcription.BC1 RNA是一种ID元件扩增主基因的转录本，能够引发自身的逆转录。

Nucleic Acids Res. 1997 Apr 15;25(8):1641-8. doi: 10.1093/nar/25.8.1641.

Structure of two human beta-actin-related processed genes one of which is located next to a simple repetitive sequence.两个人类β-肌动蛋白相关加工基因的结构，其中一个位于一个简单重复序列旁边。

EMBO J. 1983;2(5):757-61. doi: 10.1002/j.1460-2075.1983.tb01496.x.

Structure of a human beta-actin-related pseudogene which lacks intervening sequences.一个缺乏间隔序列的人类β-肌动蛋白相关假基因的结构。

Nucleic Acids Res. 1982 Dec 11;10(23):7843-9. doi: 10.1093/nar/10.23.7843.

Precursors of U4 small nuclear RNA.U4小核RNA的前体

J Cell Biol. 1984 Sep;99(3):1140-4. doi: 10.1083/jcb.99.3.1140.

Clustered genes for human U2 RNA.人类U2 RNA的成簇基因。

Proc Natl Acad Sci U S A. 1984 Jun;81(12):3811-5. doi: 10.1073/pnas.81.12.3811.

Human genes for U2 small nuclear RNA are tandemly repeated.U2小核RNA的人类基因是串联重复的。

Mol Cell Biol. 1984 Mar;4(3):492-9. doi: 10.1128/mcb.4.3.492-499.1984.

Pseudogenes for human U2 small nuclear RNA do not have a fixed site of 3' truncation.人类U2小核RNA的假基因没有固定的3'端截短位点。

Nucleic Acids Res. 1984 Feb 10;12(3):1463-71. doi: 10.1093/nar/12.3.1463.

Structural and functional analysis of chicken U4 small nuclear RNA genes.鸡U4小核RNA基因的结构与功能分析

Mol Cell Biol. 1986 Nov;6(11):3910-9. doi: 10.1128/mcb.6.11.3910-3919.1986.

Nonrandom integration of human U4 RNA pseudogenes.人类U4 RNA假基因的非随机整合

Mol Cell Biol. 1985 May;5(5):943-8. doi: 10.1128/mcb.5.5.943-948.1985.

本文引用的文献

Evidence that a human beta-tubulin pseudogene is derived from its corresponding mRNA.有证据表明人类β-微管蛋白假基因源自其相应的mRNA。

Nature. 1982 May 6;297(5861):83-4. doi: 10.1038/297083a0.

Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences.异常的类α珠蛋白基因，其已完全缺失两个珠蛋白内含子序列。

Proc Natl Acad Sci U S A. 1980 May;77(5):2806-9. doi: 10.1073/pnas.77.5.2806.

Processed genes: a dispersed human immunoglobulin gene bearing evidence of RNA-type processing.加工后的基因：一个带有RNA类型加工证据的分散人类免疫球蛋白基因。

Nature. 1982 Mar 25;296(5855):321-5. doi: 10.1038/296321a0.

Short interspersed repetitive DNA elements in eucaryotes: transposable DNA elements generated by reverse transcription of RNA pol III transcripts?真核生物中的短散在重复DNA元件：由RNA聚合酶III转录本逆转录产生的转座DNA元件？

Cell. 1981 Oct;26(2 Pt 2):141-2. doi: 10.1016/0092-8674(81)90296-8.

Origin of retroviruses from cellular moveable genetic elements.逆转录病毒起源于细胞可移动遗传元件。

Cell. 1980 Oct;21(3):599-600. doi: 10.1016/0092-8674(80)90420-1.

Transposable elements.转座元件

Cell. 1980 Jul;20(3):579-95. doi: 10.1016/0092-8674(80)90305-0.

Construction of restriction enzyme fragment libraries containing DNA from human adenovirus types 2 and 5.构建包含人2型和5型腺病毒DNA的限制性酶切片段文库。

Gene. 1980 Jun;10(1):47-52. doi: 10.1016/0378-1119(80)90142-0.

A mouse alpha-globin-related pseudogene lacking intervening sequences.一个缺乏间隔序列的小鼠α-珠蛋白相关假基因。

Nature. 1980 Jul 17;286(5770):222-6. doi: 10.1038/286222a0.

Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。

Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.

A candidate gene for human U1 RNA.人类U1 RNA的一个候选基因。

EMBO J. 1982;1(1):133-7. doi: 10.1002/j.1460-2075.1982.tb01136.x.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。