U4小核RNA的前体
Precursors of U4 small nuclear RNA.
作者信息
Madore S J, Wieben E D, Kunkel G R, Pederson T
出版信息
J Cell Biol. 1984 Sep;99(3):1140-4. doi: 10.1083/jcb.99.3.1140.
Abstract
The processing and ribonucleoprotein assembly of U4 small nuclear RNA has been investigated in HeLa cells. After a 45-min pulse label with [3H]uridine, a set of apparently cytoplasmic RNAs was observed migrating just behind the gel electrophoretic position of mature U4 RNA. These molecules were estimated to be one to at least seven nucleotides longer than mature U4 RNA. They reacted with Sm autoimmune patient sera and a monoclonal Sm antibody, indicating their association with proteins characteristic of small nuclear ribonucleoprotein complexes. The same set of RNAs was identified by hybrid selection of pulse-labeled RNA with cloned U4 DNA, confirming that these are U4 RNA sequences. No larger nuclear precursors of these RNAs were detected. Pulse-chase experiments revealed a progressive decrease in the radioactivity of the U4 precursor RNAs coincident with an accumulation of labeled mature U4 RNA, confirming a precursor-product relationship.
摘要
在HeLa细胞中研究了U4小核RNA的加工和核糖核蛋白组装。用[3H]尿苷进行45分钟脉冲标记后，观察到一组明显位于细胞质中的RNA，其迁移位置在成熟U4 RNA的凝胶电泳位置之后。估计这些分子比成熟U4 RNA长1至至少7个核苷酸。它们与Sm自身免疫患者血清和一种单克隆Sm抗体发生反应，表明它们与小核核糖核蛋白复合物的特征性蛋白质相关联。通过用克隆的U4 DNA对脉冲标记的RNA进行杂交选择，鉴定出了同一组RNA，证实这些是U4 RNA序列。未检测到这些RNA的更大的核前体。脉冲追踪实验显示，U4前体RNA的放射性逐渐降低，同时标记的成熟U4 RNA积累，证实了前体-产物关系。
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Are snRNPs involved in splicing?小核核糖核蛋白颗粒参与剪接过程吗？
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Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.体外合成的信使核糖核酸前体在HeLa细胞提取物中的剪接
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