van Putten J, van de Ruit M, Beunis M, Hemker H C
Haemostasis. 1984;14(2):195-204. doi: 10.1159/000215056.
Three automated spectrophotometric heparin assays were investigated. The day-to-day reproducibilities in routine laboratory use were compared with two commercial manual kits for heparin determination. Regression analysis of the activated partial thromboplastin time (APTT) on results of any of the heparin assays shows that the heparin concentration cannot be deduced from the APTT values found in patients receiving heparin. The automated heparin assays that employ thrombin and Chromozym-Th or S-2238 were found to be most suitable for routine heparin determination. Heparin concentrations obtained from assays based on factor Xa inactivation were not significantly different from those employing thrombin (p less than 0.01), but revealed a wider standard deviation. The relationship between APTT and heparin level found was not related to the plasma antithrombin III concentration. The extra antithrombin III that is added in the assays had to be freed of heparin neutralising activity to obtain reliable estimates of the heparin concentration in the low range (0-200 U/l).
对三种自动分光光度法肝素检测方法进行了研究。将常规实验室使用中的日间重现性与两种用于肝素测定的商业手动试剂盒进行了比较。对任何肝素检测结果进行活化部分凝血活酶时间（APTT）的回归分析表明，无法从接受肝素治疗患者的APTT值推断出肝素浓度。发现采用凝血酶和Chromozym-Th或S-2238的自动肝素检测方法最适合常规肝素测定。基于Xa因子失活的检测方法所获得的肝素浓度与采用凝血酶的方法所获得的浓度无显著差异（p小于0.01），但显示出更大的标准差。所发现的APTT与肝素水平之间的关系与血浆抗凝血酶III浓度无关。为了在低浓度范围（0 - 200 U/l）获得可靠的肝素浓度估计值，检测中添加的额外抗凝血酶III必须去除肝素中和活性。
