Bychkov V, Chejfec G
Gynecol Obstet Invest. 1984;18(1):1-5. doi: 10.1159/000299040.
The PAP immunocytochemical technique utilizing specific keratin antibody was applied to paraffin sections from 36 cervical biopsies. Normal squamous epithelium and condylomas had similar patterns of keratin production with intense staining of intermediate and upper layers, while basal cells remained negative. Dysplasia, carcinoma in situ and infiltrating squamous carcinoma showed uneven distribution of keratin with the least amount seen in the areas with high mitotic rate and anaplasia. All large cell squamous carcinomas demonstrated presence of significant amounts of keratin. Squamous carcinomas of the small cell type were essentially keratin-free.
利用特异性角蛋白抗体的PAP免疫细胞化学技术应用于36例宫颈活检的石蜡切片。正常鳞状上皮和湿疣有相似的角蛋白产生模式，中层和上层染色强烈，而基底细胞呈阴性。发育异常、原位癌和浸润性鳞状癌显示角蛋白分布不均，在有高有丝分裂率和间变的区域中含量最少。所有大细胞鳞状癌均显示存在大量角蛋白。小细胞型鳞状癌基本无角蛋白。
